Abstract:
Glycerol has been recently used to induce muscle adiposity in mice. However, its
effects on the rat muscles have not been investigated previously. Therefore, we investigated the
regeneration outcomes of rat muscles following glycerol-induced injury at different time points.
Glycerol injection induced myofiber degeneration with extensive inflammatory infiltration on
day 4 followed by appearance of regenerating myotubes on day 7 after injury without adipocyte
infiltration. Meanwhile, a significant collagen deposition at early stage of regeneration that
increased together with persistent inflammatory infiltration up to day 14 after injury indicates
impaired regeneration. In conclusion, glycerol injury in rats is more suitable as a fibrosis-inducing
model than in mice due to earlier and higher accumulation of fibrous tissue with lacking
adipogenesis.
