dc.contributor.author |
Le Roux, Marlon L.
|
|
dc.contributor.author |
Kunert, Karl J.
|
|
dc.contributor.author |
Van der Vyver, Christell
|
|
dc.contributor.author |
Cullis, Christopher A.
|
|
dc.contributor.author |
Botha, Anna-Maria
|
|
dc.date.accessioned |
2019-09-19T09:25:22Z |
|
dc.date.available |
2019-09-19T09:25:22Z |
|
dc.date.issued |
2019-03-08 |
|
dc.description.abstract |
Post-translation modification of proteins plays a critical role in cellular signaling
processes. In recent years, the SUMO (Small Ubiquitin-Like Modifier) class of molecules
has emerged as an influential mechanism for target protein management. SUMO
proteases play a vital role in regulating pathway flux and are therefore ideal targets for
manipulating stress-responses. In the present study, the expression of an Arabidopsis
thaliana cysteine protease (OVERLY TOLERANT TO SALT-1, OTS1) in wheat (Triticum
aestivum L.) has led to improved plant growth under water stress conditions.
Transformed wheat (pUBI-OTS1) displayed enhanced growth and delayed senescence
under water deficit when compared with untransformed Gamtoos-R genotype or plants
carrying an empty vector. Transformed pUBI-OTS1 plants also maintained a high relative
moisture content (RMC), had a higher photosynthesis rate, and also had a higher total
chlorophyll content when compared to untransformed plants or plants carrying an empty
vector. SUMOylation of total protein also increased in untransformed plants but not in the
AtOTS1 transformed plants. Our results suggest that SUMO-proteases may influence
an array of mechanisms in wheat to the advantage of the crop to be more tolerant to
water stress caused by drought. This is the first report to elucidate SUMOylation effects
in the hexaploid crop wheat (T. aestivum L.). |
en_ZA |
dc.description.department |
Forestry and Agricultural Biotechnology Institute (FABI) |
en_ZA |
dc.description.department |
Plant Production and Soil Science |
en_ZA |
dc.description.librarian |
am2019 |
en_ZA |
dc.description.sponsorship |
The National Research
Foundation of South Africa [NRF Competitive Programme for
Rated Researchers (CPRR) Grant: CPR20110615000019459 and NRF Incentive Funding for Rated Researchers Program (IFR)
Grant: IFR201004200013] and the Winter Cereal Trust. |
en_ZA |
dc.description.uri |
http://www.frontiersin.org/Plant_Science |
en_ZA |
dc.identifier.citation |
le Roux ML, Kunert KJ,
van der Vyver C, Cullis CA and
Botha A-M (2019) Expression of a
Small Ubiquitin-Like Modifier Protease
Increases Drought Tolerance in Wheat
(Triticum aestivum L.).
Frontiers in Plant Science 10:266.
DOI: 10.3389/fpls.2019.00266 |
en_ZA |
dc.identifier.issn |
1664-462X (online) |
|
dc.identifier.other |
10.3389/fpls.2019.00266 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/71409 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Frontiers Media |
en_ZA |
dc.rights |
© 2019 le Roux, Kunert, van der Vyver, Cullis and Botha. This is an
open-access article distributed under the terms of the Creative Commons Attribution
License (CC BY). |
en_ZA |
dc.subject |
SUMO protease |
en_ZA |
dc.subject |
Transgenic wheat |
en_ZA |
dc.subject |
Water stress tolerance |
en_ZA |
dc.subject |
Drought |
en_ZA |
dc.subject |
RuBisCo |
en_ZA |
dc.subject |
Chlorophyll fluorescence |
en_ZA |
dc.subject |
Relative moisture content (RMC) |
en_ZA |
dc.subject |
Overly tolerant to salt 1 (OTS1) |
en_ZA |
dc.subject |
Wheat (Triticum aestivum) |
en_ZA |
dc.subject |
Small ubiquitin-like modifier (SUMO) |
en_ZA |
dc.title |
Expression of a small Ubiquitin-like modifier protease increases drought tolerance in wheat (Triticum aestivum L.) |
en_ZA |
dc.type |
Article |
en_ZA |