Additional file 1: Table S1. Specifications of R. zambeziensis library
preparation procedures and the size and number of sequence reads
before and after quality filtering. Table S2. Putative R. zambeziensis
orthologues of previously characterised R. appendiculatus proteins.
Table S3. Expression proportions of the highest contributing secretory
protein families during different feeding time points. Table S4. Differential
expression analysis between female and male R. zambeziensis ticks. Table S5.
Number of differentially expressed transcripts in the protein classes and
secretory protein families of R. zambeziensis during feeding.
Additional file 2: Table S6. Annotation of the R. zambeziensis
transcriptome.
Additional file 3: Figure S1. Gene Ontology (GO) characterisation of
the R. zambeziensis transcriptome. Level 2 GO terms of cellular components,
molecular functions and biological processes were visualised using WEGO
(Web Gene Ontology Annotation Plot). These included 18,436 cellular
components, 20,487 biological processes and 9659 molecular functions.
Figure S2. KOG clustering of R. zambeziensis transcripts. In total, 9620 R.
zambeziensis transcripts were assigned to 25 Eukaryotic Clusters of
Orthologs (KOG) functional categories, of which 3814 were unique KOG
terms. Figure S3. Top 30 most abundant KEGG pathways identified in the
R. zambeziensis transcriptome. Four thousand eight hundred and sixty nine
transcripts were assigned to 338 I. scapularis Kyoto Encyclopedia of
Genes and Genomes (KEGG) pathways. Figure S4. Top 30 Pfam domain
occurrences in the R. zambeziensis predicted proteins. A total of 13,451
Pfam domains were observed in the R. zambeziensis proteins, of which
3601 were unique. Eight thousand and sixty one of the proteins contained
at least one Pfam domain.
Additional file 4: Table S7. Annotation of the predicted proteins of R.
zambeziensis.