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| dc.contributor.author | Fang, Zhuo
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| dc.contributor.author | Schubert, Wolf-Dieter
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| dc.contributor.author | Van Pittius, Nicolaas Claudius Gey
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| dc.date.accessioned | 2017-05-19T09:51:03Z | |
| dc.date.available | 2017-05-19T09:51:03Z | |
| dc.date.issued | 2016-02-06 | |
| dc.description.abstract | Mycobacteria encode five type VII secretion system (T7SS) or ESX for nutrient acquisition and virulence. Mycosins are membrane-anchored components of ESX with serine protease activity but an unidentified substrate range. Establishing the substrate specificity of individual mycosins will help to elucidate individual ESX functions. Mycosin-1 and -3 orthologues from two environmental mycobacterial species, Mycobacterium smegmatis and Mycobacterium thermoresistibile, have been heterologously produced, but mycosins from Mycobacterium tuberculosis (Mtb) remain to be studied. Here we describe the successful production of Mtb mycosin-3 as a first step in investigating its structure and function. | en_ZA |
| dc.description.department | Biochemistry | en_ZA |
| dc.description.librarian | am2017 | en_ZA |
| dc.description.sponsorship | The National Research Foundation to WDS in the form of Incentive Funding and Competitive Funding CPRR13092045495. | en_ZA |
| dc.description.uri | http://www.sherpa.ac.uk/romeo/issn/2405-5808/ | en_ZA |
| dc.identifier.citation | Fang, Z, Schubert, W-D & Van Pittus, NCG 2016, 'Expression and production of soluble Mycobacterium tuberculosis H37Rv mycosin-3', Biochemistry and Biophysics Reports, vol. 5, pp. 448-452. | en_ZA |
| dc.identifier.issn | 2405-5808 | |
| dc.identifier.other | 10.1016/j.bbrep.2016.02.005 | |
| dc.identifier.uri | http://hdl.handle.net/2263/60580 | |
| dc.language.iso | en | en_ZA |
| dc.publisher | Elsevier | en_ZA |
| dc.rights | © 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CCBY-NC-ND license | en_ZA |
| dc.subject | ESX | en_ZA |
| dc.subject | Mycosin-3 | en_ZA |
| dc.subject | Proteinproduction | en_ZA |
| dc.subject | Cloning | en_ZA |
| dc.subject | Mycobacterium tuberculosis (MTB) | en_ZA |
| dc.title | Expression and production of soluble Mycobacterium tuberculosis H37Rv mycosin-3 | en_ZA |
| dc.type | Article | en_ZA |
