dc.contributor.author |
Hawumba, J.F.
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dc.contributor.author |
Brözel, Volker Siegfried
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dc.contributor.author |
Theron, Jacques
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dc.date.accessioned |
2008-05-27T10:56:29Z |
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dc.date.available |
2008-05-27T10:56:29Z |
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dc.date.issued |
2007-12 |
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dc.description.abstract |
A 4-hydroxyphenylacetic acid (4-HPA) hydroxylase-encoding gene, on a 2.7-kb genomic DNA fragment, was cloned from the thermophile Geobacillus sp. PA-9. The Geobacillus sp. PA-9 4-HPA hydroxylase gene, designated hpaH, encodes a protein of 494 amino acids with a predicted molecular mass of 56.269 Da. The deduced amino-acid sequence of the hpaH gene product displayed <30% amino-acid sequence identity with the larger monooxygenase components of the previously characterized two-component 4-HPA 3-hydroxylases from Escherichia coli W and Klebsiella pneumoniae M5a1. A second oxidoreductase component was not present on the 2.7-kb genomic DNA fragment. The deduced amino-acid sequence of a second C-terminal truncated open reading frame, designated hpaI, exhibited homology to extradiol oxygenases and displayed the highest amino-acid sequence identity (43%) with the 3,4-dihydroxyphenylacetate 2,3-dioxygenase of Arthrobacter globiformis, encoded by mndD. These results, along with catalytic activity observed in crude intracellular extracts prepared from Escherichia coli cells expressing hpaH, is in support of a role for hpaH in the 4-HPA degradative pathway of Geobacillus sp. PA-9. |
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dc.description.sponsorship |
International Foundation for Science, the Council for Development of Social Science Research in Africa and the Makerere University Staff Development Fund. |
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dc.format.extent |
206622 bytes |
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dc.format.mimetype |
application/pdf |
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dc.identifier.citation |
Hawumba, JF, Brözel, VS & Theron, J 2007, 'Cloning and characterization of a 4-Hydroxyphenylacetate 3-Hydroxylase From the Thermophile Geobacillus sp. PA-9', Current Microbiology, vol. 55, no. 6, pp. 480-484. [www.springerlink.com] |
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dc.identifier.issn |
0343-8651 |
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dc.identifier.issn |
1432-0991 |
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dc.identifier.other |
10.1007/s00284-007-9016-5 |
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dc.identifier.uri |
http://hdl.handle.net/2263/5606 |
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dc.language.iso |
en |
en |
dc.publisher |
Springer |
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dc.rights |
Springer. The original publication is available at www.springerlink.com |
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dc.subject |
4-Hydroxyphenylacetate |
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dc.subject |
3-Hydroxylase |
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dc.subject |
Thermophile Geobacillus |
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dc.subject |
Cloning |
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dc.subject.lcsh |
Bacterial genetics |
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dc.title |
Cloning and characterization of a 4-Hydroxyphenylacetate 3-Hydroxylase from the Thermophile Geobacillus sp. PA-9 |
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dc.type |
Postprint Article |
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