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1977 Onderstepoort Journal of Veterinary Research, Volume 44, 1977: Recent submissions

  • First report of Fascioloides magna (Bassi, 1875) in South Africa 
    Dale-Kuys, June C.; Boomker, Jacob Diederik Frederik; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    Fascioloides magna from a Brahman heifer recently imported from the USA is recorded for the first time in South Africa. The pathological findings are briefly described. The possibility of this parasite becoming established ...
  • Effect of levamisole on immunity to Corynebacterium pseudotuberculosis in mice and sheep 
    Cameron, Colin McKenzie; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    The stimulating effect of levamisole on immunity to Corynebacterium pseudotuberculosis in mice was marginal, while no enhancement of immunity could be detected in sheep. The conclusion was reached that, as levamisole has ...
  • Cryopreservation of the infective larvae of the common nematodes of ruminants 
    Van Wyk, Jan Aucamp; Gerber, H.M.; Van Aardt, W.P.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    Exsheathed infective larvae (L 3) of 19 species of nematodes were tested for infectivity in either sheep or cattle after they had been frozen in 0, 9% NaCI solution, stored for a relatively short time in the gas phase of ...
  • Studies on Parafilaria bovicola Tubangui, 1934 : 2. Chemotherapy and pathology 
    Viljoen, J.H.; Boomker, Jacob Diederik Frederik; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (The Government Printer, Pretoria, 1977)
    The filaricidal effects of trichlorphon, arsenic trioxide, sodium antimony biscathecol disulphonate and nitroxynil against Parafilaria bovicola were the subject of this investigation . Levamisole hydrochloride was retested ...
  • An accurate, sensitive and reproducible method for colorimetric estimation of free fatty acids in plasma 
    De Villiers, Saramarie; Van der Walt, J.G.; Procos, J.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    A sensitive and reproducible method permitting the estimation of 0,030-3,000 mM of free fatty acids (FFA) in plasma with a standard error of 0,006 5 was developed. The method is based on the extraction of phosphate-buffered ...
  • Disseminated protothecosis in a dog 
    Imes, G. D.; Lloyd, June C.; Brightman, M. P.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    This is a report on the clinical history and pathological lesions of a dog suffering from disseminated protothecosis due to Prototheca zopfii. Clinically, the dog was presented with bilateral conjunctivitis followed by ...
  • The separation of some volatile fatty acids on a "Sephadex" partition chromatogram 
    Van der Walt, J.G.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (The Government Printer,Pretoria, 1977)
    The suitability of Sephadex LH-20 as a solid support for the partition chromatography of acetic, propionic and butyric acids was investigated by using standard mixtures of 14C-labelled acids. The clear separation that was ...
  • Ixodes (Afrixodes) neitzi, n. sp. (Acarina :Ixo didae) from the mountain reedbuck in South Africa 
    Clifford, C.M.; Walker, Jane B.; Keirans, J.E.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    Ixodes (Afrixodes) neitzi n. sp. is described from females and males collected from the mountain reedbuck (Redunca fulvorufula) at Loskop Dam Nature Reserve, Transvaal, Republic of South Africa. Information is provided to ...
  • Sheep erythrocyte and bluetongue virus antibody responses of spleen cell cultures from mice 
    Carter, P.; Oellermann, R.A.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Government Printer, Pretoria, 1977)
    The optimum conditions for the culture of cells from dissociated spleens were determined. Routinely, 10⁷ cells were seeded per ml of RPMI 1640 medium supplemented with 20% pre-tested foetal calf serum. For the assay of the ...
  • Parasites of domestic and wild animals in South Africa. II. Oestrus ovis in goats 
    Horak, Ivan Gerard; Butt, M.J.; De Kock, V.E.; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Bigalke, R.D.; Cameron, Colin McKenzie; Walker, Jane B. (The Government Printer,Pretoria, 1977)
    During a period of 13 months, 130 goats' heads, obtained from the Johannesburg Municipal Abattoir, were examined for infestation with Oestrus ovis. Infestation was present in 73,8% of the heads and the mean burden comprised ...
  • The arterial blood supply of the cervical vertebrae of the ox (Bos taurus L.) 
    Smuts, M.M.S. (Malie Marie Sophie), 1933-; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    The arterial supply to the cervical vertebrae of the ox was studied in 22 animals (Friesland, Jersey and Guernsey cross-breeds), ranging from near full-term foetuses to adults. Various techniques, including angiography and ...
  • The fine structure of intra-erythrocytic stages of Babesia bigemina 
    Potgieter, F.T.; Els, H.J.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    The electron microscope was used to study the structure of merozoites, merozoites in the process of transformation to trophozoites, trophozoites, and the method of multiplication of B. bigemina. The merozoites were piriform ...
  • Negative staining of a non-haemadsorbing strain of African swine fever virus 
    Els, H.J.; Pini, A.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    Since the application of negative staining, preceded by fixation, prevents the disruption and distortion of the capsid of the African swine fever virus, improved contrast and evaluation of the appearance and size of virus ...
  • Gamma-glutamyl transpeptidase activity in sheep serum : normal values and an evaluation of its potential for detecting liver involvement in experimental lupinosis 
    Malherbe, W.D.; Kellerman, T.S.; Kriek, N.P.J.; Haupt, W.H.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    A brief survey of the literature on gamma-glutamyl transpeptidase (ƴ-GT) activity is included in this study. The levels of activity in the serum of normal Merino sheep (13, 6- 32, 4 mI.U./ml) were ascertained as a preliminary ...
  • Immunosuppression in new-born lambs 
    Broekman, J.; Eksteen, P.A.L.; Verwoerd, Daniel Wynand; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    A novel technique, based on cytotoxicity-neutralization, was developed for the in vitro titration of anti-sheep lymphocyte and anti-sheep macrophage sera. The titres obtained for a number of antisera were compared with ...
  • The life cycle of the two-host tick Rhipicephalus evertsi evertsi Neumann, 1897, under laboratory conditions (Acarina : Ixodidae) 
    Londt, J.G.H.; Van der Bijl, Eleanore B.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Pretoria : The Government Printer, 1977)
    The life cycle of Rhipicephalus evertsi evertsi Neumann, 1897, is discussed under the following headings: larval feeding; larval drop; nymphal feeding; nymphal drop; nymphal moulting; adult feeding; adult drop; preoviposition ...
  • Studies on Schistosomiasis. 9. Pathology of the bovine urinary tract 
    Bartsch, R.C.; Van Wyk, Jan Aucamp; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (The Government Printer, Pretoria, 1977)
    Pathological findings in the urinary tract of 40 head of cattle, experimentally or naturally infested with Schistosoma mattheei, are presented. Lesions of the ureter found in 57,5% of the cases consisted of linear granulomata ...
  • Oviposition and incubation in Boophilus decoloratus (Koch, 1844) (Acarina : Ixodidae) 
    Londt, J.G.H.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (The Government Printer, Pretoria, 1977)
    Studies on the duration of the oviposition and incubation periods of Boophilus decoloratus (Koch, 1844) revealed that these non-parasitic periods are temperature dependent, increase in temperature causing shortening of the ...
  • Volatile fatty acid metabolism in sheep. 2. Correlation between the volatile fatty acid production and concentration in the rumen during the course of a feeding cycle 
    Van der Walt, J. G.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (The Government Printer, Pretoria, 1977)
    Four German Merino wethers were maintained on 1600 g of lucerne hay fed twice daily. A dilute solution of a labelled volatile fatty acid (VFA) marker was infused over a period of 22 h and 6 discrete rumen fluid samples ...