An accurate, sensitive and reproducible method for colorimetric estimation of free fatty acids in plasma
De Villiers, Saramarie; Van der Walt, J.G.; Procos, J.; De Kock, V.E.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.
A sensitive and reproducible method permitting the estimation of 0,030-3,000 mM of free fatty acids (FFA) in plasma with a standard error of 0,006 5 was developed. The method is based on the extraction of phosphate-buffered plasma with a chloroform-heptane-methanol mixture, formation of the cobalt complex and on the subsequent determination of the metal with 1-nitroso-2 naphthol. The method was used to obtain normal values (mean 0,547 mM range 0,147-1,197 mM) for Merino wethers fed lucerne hay.
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