The effect of incubation and prefeeding of infected Rhipicephalus simus nymphae and adults on the transmission of Anaplasma marginale
Potgieter, F.T.; Van Rensburg, L.; Steyn, P.J.J.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Jordaan, E.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.
Two batches of unfed Rhipicephalus simus nymphae carrying Anaplasma marginale were incubated for 72h and 96h respectively at 37°C. Fifty ticks were triturated at a time and the homogenates were used to infect susceptible cattle. Others were prefed on a bovine host for 72h before tick suspensions were prepared. The same procedure was followed, using a single batch of infected adult ticks.
Incubation had no effect on the infectivity of the nymphal homogenates, but prefeeding of nymphae reduced the prepatent period by 8 days in l of the 2 attempts. In the case of the adult ticks both incubation and prefeeding reduced prepatent periods by 5 and 8 days respectively.
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