The use of the serum agglutination test (SAT) as a 2nd screening test led to a reduction in false negative
screening reactions especially in sera delayed in transit and provided an easy check on human error in the rose
bengal test (RBT) and on each individual complement fixation test titre. The SAT in microtitration plates was
more sensitive than when done in tubes and effected a saving of time, labour and materials.
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