dc.contributor.author |
Morreel, Kris
|
|
dc.contributor.author |
Saeys, Yvan
|
|
dc.contributor.author |
Dima, Oana
|
|
dc.contributor.author |
Lu, Fachuang
|
|
dc.contributor.author |
Van de Peer, Yves
|
|
dc.contributor.author |
Vanholme, Ruben
|
|
dc.contributor.author |
Ralph, John
|
|
dc.contributor.author |
Vanholme, Bartel
|
|
dc.contributor.author |
Boerjan, Wout
|
|
dc.date.accessioned |
2015-09-04T07:21:37Z |
|
dc.date.available |
2015-09-04T07:21:37Z |
|
dc.date.issued |
2014-03 |
|
dc.description.abstract |
Plant metabolomics is increasingly used for pathway discovery and to elucidate gene function. However, the main bottleneck
is the identification of the detected compounds. This is more pronounced for secondary metabolites as many of their
pathways are still underexplored. Here, an algorithm is presented in which liquid chromatography–mass spectrometry
profiles are searched for pairs of peaks that have mass and retention time differences corresponding with those of substrates
and products from well-known enzymatic reactions. Concatenating the latter peak pairs, called candidate substrate-product
pairs (CSPP), into a network displays tentative (bio)synthetic routes. Starting from known peaks, propagating the network
along these routes allows the characterization of adjacent peaks leading to their structure prediction. As a proof-of-principle,
this high-throughput cheminformatics procedure was applied to the Arabidopsis thaliana leaf metabolome where it allowed
the characterization of the structures of 60% of the profiled compounds. Moreover, based on searches in the Chemical
Abstract Service database, the algorithm led to the characterization of 61 compounds that had never been described in plants
before. The CSPP-based annotation was confirmed by independent MSn experiments. In addition to being high throughput,
this method allows the annotation of low-abundance compounds that are otherwise not amenable to isolation and
purification. This method will greatly advance the value of metabolomics in systems biology. |
en_ZA |
dc.description.librarian |
hb2015 |
en_ZA |
dc.description.sponsorship |
Stanford University Global Climate and Energy Project (“Towards New Degradable Lignin Types,” “Efficient Biomass Conversion: Delineating the Best Lignin Monomer-Substitutes,” and “Lignin management: optimizing yield and composition in lignin-modified plants”) by the Multidisciplinary Research Project “Biotechnology for a sustainable economy” of Ghent University, by the European Community’s Seventh
Framework Programme (FP7/2009) under grant agreement 251132 (SUNLIBB),
and the “Bijzonder Onderzoeksfonds-ZwareApparatuur” of Ghent
University for the FT-ICR-MS (Grant 174PZA05).Research Foundation-Flanders. |
en_ZA |
dc.description.uri |
http://www.plantcell.org |
en_ZA |
dc.identifier.citation |
Morreel, K, Saeys, Y, Dima, O, Lu, F, Van de Peer, Y, Vanholme, R, Ralph, J, Vanholme, B & Boerjan, W 2014, 'Systematic structural characterization of metabolites in arabidopsis via candidate substrate-product pair networks', Plant Cell, vol. 26, no. 3, pp. 929-945. |
en_ZA |
dc.identifier.issn |
1040-4651 (print) |
|
dc.identifier.issn |
1532-298X (online) |
|
dc.identifier.other |
10.1105/tpc.113.122242 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/49708 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
American Society of Plant Biologists |
en_ZA |
dc.rights |
© 2014 American Society of Plant Biologists. All rights reserved. |
en_ZA |
dc.subject |
Structural characterization |
en_ZA |
dc.subject |
Metabolites in arabidopsis |
en_ZA |
dc.subject |
Plant metabolomics |
en_ZA |
dc.subject |
Pathway discovery |
en_ZA |
dc.subject |
Detected compounds |
en_ZA |
dc.subject |
Candidate substrate-product pairs (CSPP) |
en_ZA |
dc.title |
Systematic structural characterization of metabolites in arabidopsis via candidate substrate-product pair networks |
en_ZA |
dc.type |
Postprint Article |
en_ZA |