Techno-economic analysis of horseradish peroxidase production using a transient expression system in Nicotiana benthamiana

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dc.contributor.author Walwyn, David Richard
dc.contributor.author Huddy, Suzanne M.
dc.contributor.author Rybicki, Edward P.
dc.date.accessioned 2015-02-02T09:53:31Z
dc.date.available 2015-02-02T09:53:31Z
dc.date.issued 2015-01
dc.description.abstract Despite the advantages of plant-based transient expression systems relative to microbial or mammalian cell systems, the commercial production of recombinant proteins using plants has not yet been achieved to any significant extent. One of the challenges has been the lack of published data on the costs of manufacture for products other than biopharmaceuticals. In this study, we report on the techno-economic analysis of the production of a standard commercial enzyme, namely, horseradish peroxidase (HRP), using a transient expression system in Nicotiana benthamiana. Based on the proven plant yield of 240 mg HRP/kg biomass, a biomass productivity of 15-kg biomass/m2/year and a process yield of 54 % (mg HRP product/mg HRP in biomass), it is apparent that HRP can be manufactured economically via transient expression in plants in a large-scale facility (>5 kg HRP/year). At this level, the process is competitive versus the existing technology (extraction of the enzyme from horseradish), and the product is of comparable or improved activity, containing only the preferred isoenzyme C. Production scale, protein yield and biomass productivity are found to be the most important determinants of overall viability. en_ZA
dc.description.embargo 2016-01-30 en_ZA
dc.description.librarian hj2015 en_ZA
dc.description.sponsorship The National Intellectual Property Management Organisation, the Research Contracts and Intellectual Property Services of the University of Cape Town and the Technology Innovation Agency and the Department of Science and Technology. en_ZA
dc.description.uri http://link.springer.com/journal/12010 en_ZA
dc.identifier.citation Walwyn, DR, Huddy, SM & Rybicki, EP 2014, 'Techno-economic analysis of horseradish peroxidase production using a transient expression system in Nicotiana benthamiana', Applied Biochemistry and Biotechnology, vol. 175, no. 2, pp. 841-854. en_ZA
dc.identifier.issn 0273-2289 (print)
dc.identifier.issn 1559-0291 (online)
dc.identifier.other 10.1007/s12010-014-1320-5
dc.identifier.uri http://hdl.handle.net/2263/43516
dc.language.iso en en_ZA
dc.publisher Springer en_ZA
dc.rights © Springer Science+Business Media New York 2014. The original publication is available at : http://link.springer.comjournal/12010 en_ZA
dc.subject Techno-economic analysis en_ZA
dc.subject Horseradish peroxidase (HRP) en_ZA
dc.subject Transient expression en_ZA
dc.subject Agroinfiltration en_ZA
dc.subject Commercial production en_ZA
dc.subject Nicotiana benthamiana en_ZA
dc.title Techno-economic analysis of horseradish peroxidase production using a transient expression system in Nicotiana benthamiana en_ZA
dc.type Postprint Article en_ZA


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