dc.contributor.author |
Walwyn, David Richard
|
|
dc.contributor.author |
Huddy, Suzanne M.
|
|
dc.contributor.author |
Rybicki, Edward P.
|
|
dc.date.accessioned |
2015-02-02T09:53:31Z |
|
dc.date.available |
2015-02-02T09:53:31Z |
|
dc.date.issued |
2015-01 |
|
dc.description.abstract |
Despite the advantages of plant-based transient expression systems relative to
microbial or mammalian cell systems, the commercial production of recombinant proteins
using plants has not yet been achieved to any significant extent. One of the challenges has been
the lack of published data on the costs of manufacture for products other than
biopharmaceuticals. In this study, we report on the techno-economic analysis of the production
of a standard commercial enzyme, namely, horseradish peroxidase (HRP), using a transient
expression system in Nicotiana benthamiana. Based on the proven plant yield of 240 mg
HRP/kg biomass, a biomass productivity of 15-kg biomass/m2/year and a process yield of
54 % (mg HRP product/mg HRP in biomass), it is apparent that HRP can be manufactured
economically via transient expression in plants in a large-scale facility (>5 kg HRP/year). At
this level, the process is competitive versus the existing technology (extraction of the enzyme
from horseradish), and the product is of comparable or improved activity, containing only the
preferred isoenzyme C. Production scale, protein yield and biomass productivity are found to
be the most important determinants of overall viability. |
en_ZA |
dc.description.embargo |
2016-01-30 |
en_ZA |
dc.description.librarian |
hj2015 |
en_ZA |
dc.description.sponsorship |
The National Intellectual Property Management Organisation, the
Research Contracts and Intellectual Property Services of the University of Cape Town and the Technology Innovation Agency and the Department of Science and Technology. |
en_ZA |
dc.description.uri |
http://link.springer.com/journal/12010 |
en_ZA |
dc.identifier.citation |
Walwyn, DR, Huddy, SM & Rybicki, EP 2014, 'Techno-economic analysis of horseradish peroxidase production using a transient expression system in Nicotiana benthamiana', Applied Biochemistry and Biotechnology, vol. 175, no. 2, pp. 841-854. |
en_ZA |
dc.identifier.issn |
0273-2289 (print) |
|
dc.identifier.issn |
1559-0291 (online) |
|
dc.identifier.other |
10.1007/s12010-014-1320-5 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/43516 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Springer |
en_ZA |
dc.rights |
© Springer Science+Business Media New York 2014. The original publication is available at : http://link.springer.comjournal/12010 |
en_ZA |
dc.subject |
Techno-economic analysis |
en_ZA |
dc.subject |
Horseradish peroxidase (HRP) |
en_ZA |
dc.subject |
Transient expression |
en_ZA |
dc.subject |
Agroinfiltration |
en_ZA |
dc.subject |
Commercial production |
en_ZA |
dc.subject |
Nicotiana benthamiana |
en_ZA |
dc.title |
Techno-economic analysis of horseradish peroxidase production using a transient expression system in Nicotiana benthamiana |
en_ZA |
dc.type |
Postprint Article |
en_ZA |