Techno-economic analysis of horseradish peroxidase production using a transient expression system in Nicotiana benthamiana

dc.contributor.authorWalwyn, David Richard
dc.contributor.authorHuddy, Suzanne M.
dc.contributor.authorRybicki, Edward P.
dc.contributor.emaildavid.walwyn@up.ac.zaen_ZA
dc.date.accessioned2015-02-02T09:53:31Z
dc.date.available2015-02-02T09:53:31Z
dc.date.issued2015-01
dc.description.abstractDespite the advantages of plant-based transient expression systems relative to microbial or mammalian cell systems, the commercial production of recombinant proteins using plants has not yet been achieved to any significant extent. One of the challenges has been the lack of published data on the costs of manufacture for products other than biopharmaceuticals. In this study, we report on the techno-economic analysis of the production of a standard commercial enzyme, namely, horseradish peroxidase (HRP), using a transient expression system in Nicotiana benthamiana. Based on the proven plant yield of 240 mg HRP/kg biomass, a biomass productivity of 15-kg biomass/m2/year and a process yield of 54 % (mg HRP product/mg HRP in biomass), it is apparent that HRP can be manufactured economically via transient expression in plants in a large-scale facility (>5 kg HRP/year). At this level, the process is competitive versus the existing technology (extraction of the enzyme from horseradish), and the product is of comparable or improved activity, containing only the preferred isoenzyme C. Production scale, protein yield and biomass productivity are found to be the most important determinants of overall viability.en_ZA
dc.description.embargo2016-01-30en_ZA
dc.description.librarianhj2015en_ZA
dc.description.sponsorshipThe National Intellectual Property Management Organisation, the Research Contracts and Intellectual Property Services of the University of Cape Town and the Technology Innovation Agency and the Department of Science and Technology.en_ZA
dc.description.urihttp://link.springer.com/journal/12010en_ZA
dc.identifier.citationWalwyn, DR, Huddy, SM & Rybicki, EP 2014, 'Techno-economic analysis of horseradish peroxidase production using a transient expression system in Nicotiana benthamiana', Applied Biochemistry and Biotechnology, vol. 175, no. 2, pp. 841-854.en_ZA
dc.identifier.issn0273-2289 (print)
dc.identifier.issn1559-0291 (online)
dc.identifier.other10.1007/s12010-014-1320-5
dc.identifier.urihttp://hdl.handle.net/2263/43516
dc.language.isoenen_ZA
dc.publisherSpringeren_ZA
dc.rights© Springer Science+Business Media New York 2014. The original publication is available at : http://link.springer.comjournal/12010en_ZA
dc.subjectTechno-economic analysisen_ZA
dc.subjectHorseradish peroxidase (HRP)en_ZA
dc.subjectTransient expressionen_ZA
dc.subjectAgroinfiltrationen_ZA
dc.subjectCommercial productionen_ZA
dc.subjectNicotiana benthamianaen_ZA
dc.titleTechno-economic analysis of horseradish peroxidase production using a transient expression system in Nicotiana benthamianaen_ZA
dc.typePostprint Articleen_ZA

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Walwyn_Techno_2015.pdf
Size:
1.31 MB
Format:
Adobe Portable Document Format
Description:
Postprint Article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: