In vitro cultivation of a Babesia sp. from cattle in South Africa

Show simple item record Van Niekerk, C. Just, M.C. De Waal, D.T.
dc.contributor.editor Verwoerd, Daniel Wynand
dc.contributor.upauthor Zweygarth, Erich 2013-09-12T08:07:45Z 2013-09-12T08:07:45Z 2013 1995
dc.description The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. en
dc.description.abstract A South African Babesia sp. of cattle which is as yet unclassified, was continuously cultivated in micro-aerophilous stationary-phase culture. The parasites were resuscitated from a blood stabilate stored in liquid nitrogen. A modified HL-1 medium supplemented with either horse or bovine serum was used. Cultures were initiated in a humidified atmosphere containing 2% 0₂ , 5% C0₂ and 93% N₂ at 37°C. Parasites were detected on Giemsa-stained smears after 2 d in culture. On day 4, the cultures were split at a ratio of 1:2 (v/v) and transferred into a humidified atmosphere of 5% C0₂ in air. Starting from day 6, subcultures were made daily at a ratio of 1:4 (v/v). The percentage of parasitized erythrocytes ranged from 2-5%. Addition of purine bases (hypoxanthine, adenine, adenosine or guanosine) was essential for the continuous propagation of the parasites when bovine, but not horse serum, was used for medium supplementation. en
dc.description.librarian mn2013
dc.identifier.citation Zweygarth, E, Van Niekerk, C, Just, MC & De Waal, DT 1995, 'In vitro cultivation of a Babesia sp. from cattle in South Africa’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 2, pp. 139-142. en
dc.identifier.issn 0330-2465
dc.language.iso en en
dc.publisher Published by the Agricultural Research Council, Onderstepoort Veterinary Institute en
dc.rights © ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital). en
dc.subject Veterinary medicine en
dc.subject In vitro en
dc.subject Cultivation en
dc.subject Babesia en
dc.subject Cattle en
dc.subject Bovine en
dc.subject South Africa en
dc.subject.lcsh Veterinary medicine -- South Africa
dc.title In vitro cultivation of a Babesia sp. from cattle in South Africa en
dc.type Article en

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