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dc.contributor.author | Van Niekerk, C. | |
dc.contributor.author | Just, M.C. | |
dc.contributor.author | De Waal, D.T. | |
dc.contributor.editor | Verwoerd, Daniel Wynand | |
dc.contributor.upauthor | Zweygarth, Erich | |
dc.date.accessioned | 2013-09-12T08:07:45Z | |
dc.date.available | 2013-09-12T08:07:45Z | |
dc.date.created | 2013 | |
dc.date.issued | 1995 | |
dc.description | The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. | en |
dc.description.abstract | A South African Babesia sp. of cattle which is as yet unclassified, was continuously cultivated in micro-aerophilous stationary-phase culture. The parasites were resuscitated from a blood stabilate stored in liquid nitrogen. A modified HL-1 medium supplemented with either horse or bovine serum was used. Cultures were initiated in a humidified atmosphere containing 2% 0₂ , 5% C0₂ and 93% N₂ at 37°C. Parasites were detected on Giemsa-stained smears after 2 d in culture. On day 4, the cultures were split at a ratio of 1:2 (v/v) and transferred into a humidified atmosphere of 5% C0₂ in air. Starting from day 6, subcultures were made daily at a ratio of 1:4 (v/v). The percentage of parasitized erythrocytes ranged from 2-5%. Addition of purine bases (hypoxanthine, adenine, adenosine or guanosine) was essential for the continuous propagation of the parasites when bovine, but not horse serum, was used for medium supplementation. | en |
dc.description.librarian | mn2013 | |
dc.identifier.citation | Zweygarth, E, Van Niekerk, C, Just, MC & De Waal, DT 1995, 'In vitro cultivation of a Babesia sp. from cattle in South Africa’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 2, pp. 139-142. | en |
dc.identifier.issn | 0330-2465 | |
dc.identifier.uri | http://hdl.handle.net/2263/31679 | |
dc.language.iso | en | en |
dc.publisher | Published by the Agricultural Research Council, Onderstepoort Veterinary Institute | en |
dc.rights | © ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital). | en |
dc.subject | Veterinary medicine | en |
dc.subject | In vitro | en |
dc.subject | Cultivation | en |
dc.subject | Babesia | en |
dc.subject | Cattle | en |
dc.subject | Bovine | en |
dc.subject | South Africa | en |
dc.subject.lcsh | Veterinary medicine -- South Africa | |
dc.title | In vitro cultivation of a Babesia sp. from cattle in South Africa | en |
dc.type | Article | en |