dc.contributor.author |
Van Niekerk, C.
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dc.contributor.author |
Just, M.C.
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|
dc.contributor.author |
De Waal, D.T.
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dc.contributor.editor |
Verwoerd, Daniel Wynand |
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dc.contributor.upauthor |
Zweygarth, Erich
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dc.date.accessioned |
2013-09-12T08:07:45Z |
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dc.date.available |
2013-09-12T08:07:45Z |
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dc.date.created |
2013 |
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dc.date.issued |
1995 |
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dc.description |
The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. |
en |
dc.description.abstract |
A South African Babesia sp. of cattle which is as yet unclassified, was continuously cultivated in micro-aerophilous stationary-phase culture. The parasites were resuscitated from a blood stabilate stored in
liquid nitrogen. A modified HL-1 medium supplemented with either horse or bovine serum was used.
Cultures were initiated in a humidified atmosphere containing 2% 0₂ , 5% C0₂ and 93% N₂ at 37°C.
Parasites were detected on Giemsa-stained smears after 2 d in culture. On day 4, the cultures were
split at a ratio of 1:2 (v/v) and transferred into a humidified atmosphere of 5% C0₂ in air. Starting from
day 6, subcultures were made daily at a ratio of 1:4 (v/v). The percentage of parasitized erythrocytes
ranged from 2-5%. Addition of purine bases (hypoxanthine, adenine, adenosine or guanosine) was
essential for the continuous propagation of the parasites when bovine, but not horse serum, was used
for medium supplementation. |
en |
dc.description.librarian |
mn2013 |
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dc.identifier.citation |
Zweygarth, E, Van Niekerk, C, Just, MC & De Waal, DT 1995, 'In vitro cultivation of a Babesia sp. from cattle in South Africa’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 2, pp. 139-142. |
en |
dc.identifier.issn |
0330-2465 |
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dc.identifier.uri |
http://hdl.handle.net/2263/31679 |
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dc.language.iso |
en |
en |
dc.publisher |
Published by the Agricultural Research Council, Onderstepoort Veterinary Institute |
en |
dc.rights |
© ARC-Onderstepoort (original).
© University of Pretoria. Dept of Library Services (digital). |
en |
dc.subject |
Veterinary medicine |
en |
dc.subject |
In vitro |
en |
dc.subject |
Cultivation |
en |
dc.subject |
Babesia |
en |
dc.subject |
Cattle |
en |
dc.subject |
Bovine |
en |
dc.subject |
South Africa |
en |
dc.subject.lcsh |
Veterinary medicine -- South Africa |
|
dc.title |
In vitro cultivation of a Babesia sp. from cattle in South Africa |
en |
dc.type |
Article |
en |