• The diagnosis of Wesselsbron disease in a new-born lamb by immunohistochemical staining of viral antigen 

  Coetzer, Jacobus A.W.; Van der Lugt, Jaco J.; Cilliers, C.; Smit, Marie M.E.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  Wesselsbron disease (WSL) was diagnosed in a 2-d-old lamb on a farm in the north-eastern Free State Province where a few abortions and neonatal deaths occurred in sheep in April 1994. The liver of the lamb was slightly ...
• Epidemiology of African horsesickness: antibodies in free-living elephants (Loxodonta africana) and their response to experimental infection 

  Barnard, B.J.H.; Bengis, Roy G.; Keet, D.F.; Dekker, E.H.; Verwoerd, Daniel Wynand (Pretoria : Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  The presence of low levels of group- and type-specific antibodies against African horsesickness virus in the serum of some free-living elephants was reconfirmed. Experimental infection resulted in conflicting results. No ...
• Author index 

  Verwoerd, Daniel Wynand (Pretoria : Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)
• The use of sucrose-acetone-extracted Rift Valley fever virus antigen derived from cell culture in an indirect enzyme-linked immunosorbent assay and haemagglutination-inhibition test 

  Paweska, Janusz Tadeusz; Barnard, B.J.H.; Williams, R.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  A sucrose-acetone-extracted, Madin-Darby-bovine-kidney (MDBK)-derived Rift Valley fever virus (RVFV) antigen was tested both in an indirect ELISA and a haemagglutination-inhibition test for its ability to detect serum ...
• The distribution of Pasteurella haemolytica serotypes among cattle, sheep and goats in South Africa and their association with disease 

  Odendaal, M.W.; Henton, Marijke M.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  Over an 8-year period (September 1986 to March 1994), a total of 497 organ specimens from sheep and goats and 96 from cattle, were received for the isolation of Pasteurella haemolytica. They were collected in seven ...
• Subject index 

  Anonymous; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)
• An improved technique for the cryopreservation of Gaigeria pachyscelis (Sandveld hookworm) 

  Titoy, G.A.P.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  A technique for the cryopreservation of third-stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed infective larvae at 37°C in 40% (v/v) ethylene glycol for 7 min, followed by 2 min at 0°C ...
• Monitoring experimental Alcelaphine Herpesvirus-1 infection in cattle by nucleic-acid hybridization and PCR 

  Michel, Anita Luise; Buchholz, G.S.; Van der Lugt, Jaco J.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  The DNA probe SW15 derived from the laboratory-attenuated Alcelaphine Herpesvirus-1 (AHV-1) strain WC11 as well as from the polymerase chain-reaction test (Hsu, Shih, Castro & Zee 1990), was used to detect viral DNA of ...
• Microclimatic effect on vertical migration of Haemonchus contortus and Haemonchus placei third-stage larvae on irrigated Kikuyu pasture 

  Groeneveld, Hendrik T.; Krecek, R.C.; Hartman, R.; Thorne, A.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  The influence of microclimate on numbers of third-stage larvae of Haemonchus contortus and Haemonchus placei in four strata of irrigated Kikuyu pasture was assessed. On 36 different, interspersed days three replicates of ...
• An outbreak of encephalomyocarditis-virus infection in free-ranging African elephants in the Kruger National Park 

  Braack, L.E.O.; Grobler, D.G.; Raath, J.P.; Keet, D.F.; Gerdes, Gertruida Hermanna; Barnard, B.J.H.; Kriek, N.P.J.; Jardine, J.; Swanepoel, R.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  A cluster of four deaths in late December 1993, marked the onset of an outbreak of disease of African elephants (Loxodonta africana) in the Kruger National Park (KNP) in South Africa, which has an estimated population ...
• Rhipicephalus interventus sp. nov. (Acari: Ixodidae), a new tick species closely related to Rhipicephalus tricuspis Dönitz, 1906 and Rhipicephalus lunulatus Neumann, 1907, from East and Central Africa 

  Walker, Jane B.; Keirans, J.E.; Pegram, R.G.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  Descriptions of the adults of this new species are given, together with information on its hosts and distribution. Previously it was referred to briefly by Walker, Keirans, Pegram & Clifford (1988), who noted that in ...
• Isolation of the toxin responsible for gousiekte, a plant-induced cardiomyopathy of ruminants in southern Africa 

  Prozesky, Leon; Fourie, Niel; Erasmus, G.L.; Schultz, R.A. (Rowena Anitra); Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  Gousiekte was induced in sheep and goats by the oral and intravenous (i/v) administration of methanolic and ethanolic precipitates of aqueous extracts of Pavetta harborii. Further purification of the toxic principle was ...
• The skull and mandible of the African elephant (Loxodonta africana) 

  Van der Merwe, N.J.; Bezuidenhout, A.J. (Abraham Johannes), 1942-; Seegers, Christine D.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  In the present study the bones of the skull, excluding the hyoid apparatus, are described. All the bones are aerated by sinuses. In the occipital bone the squamous part is aerated from the sinus of the parietal bone, the ...
• Guide for authors 

  Verwoerd, Daniel Wynand (Pretoria : Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)
• Fleas, lice and mites on scrub hares (Lepus saxatilis) in Northern and Eastern Transvaal and in KwaZulu-Natal, South Africa 

  Horak, Ivan Gerard; Braack, L.E.O.; Louw, J.P.; Horak, M. Louise; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  Fleas, lice and mites were collected from 24 and 120 scrub hares at Pafuri and Skukuza, Northern and Eastern Transvaal, respectively, in the Kruger National Park, and from 34 scrub hares in the Hluhluwe region, north-eastern ...
• Immunohistochemical identification of Cowdria ruminantium in formalin-fixed tissue sections from mice, sheep, cattle and goats 

  Van Kleef, Mirinda; Jardine, J.E.; Vogel, S.W.; Van der Lugt, Jaco J.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  An immunohistochemical staining technique in which a monospecific serum was used against the major antigenic protein-1 (MAP-1) of Cowdria ruminantium, was evaluated for the detection of C. ruminantium in formalin-fixed ...
• Detection of bovine-virus-diarrhoea-virus antibodies in cattle with an enzyme-linked immunosorbent assay 

  Muvavarirwa, P.; Mudenge, D.; Moyo, Doreen Zandile; Javangwe, S.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  The serum-neutralization (SN) and the indirect-immunofluorescence (IIF) assays have invariably been used for detecting antibodies against bovine virus diarrhoea virus (BVDV) in cattle sera. An enzyme-linked immunosorbent ...
• The effect(s) of carbaryl-treated seed on body maintenance and survival of the multi-mammate mouse, Mastomys natalensis (sensu lato) 

  Van Aarde, Rudi J.; Neluvhalani, M.J.; Ferreira, Sam M.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  Maintenance, expressed as change in daily body mass, and survival rates of Mastomys natalensis (sensu lato) were recorded from May to September 1994 in laboratory feeding trials, to investigate the short-term effects of a ...
• Effects of transformation on the hemagglutinins of Haemophilus paragallinarum 

  Verschoor, J.A. (Jan Adrianus), 1953-; Bragg, R.R. (Robert Richard); Purdan, G.; Coetzee, L.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  Strain 0083 and two field isolates of H. paragallinarum were previously converted into NAD-independent organisms by the use of crude DNA extractions from naturally occurring NAD-independent H. paragallinarumisolates. Two ...
• Parasites of domestic and wild animals in South Africa. XXXIII. Ixodid ticks on scrub hares in the north-eastern regions of Northern and Eastern Transvaal and of KwaZulu-Natal 

  Horak, Ivan Gerard; Braack, L.E.O.; Penzhorn, Barend Louis; Spickett, Arthur M.; Bagnall, R.J.; Uys, A.C. (André Charles); Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)

  Ixodid ticks were collected from scrub hares (Lepus saxatilis) at three localities. Nine tick species were recovered from 24 hares examined at Pafuri, Kruger National Park, Northern Transvaal. The most abundant and ...

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