A technique for the cryopreservation of sheathed third-stage larvae of Nematodirus spathiger is described.
It consists of incubating larvae in 5% (v/v) ethylene glycol for 20 min at room temperature,
followed by 1 min of incubation in 40% (v/v) ethylene glycol at 0°C. The average recovery of adult worms
was 28,5% for two adult sheep infected with these larvae.
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