dc.contributor.author |
Maritz-Olivier, Christine
|
|
dc.contributor.author |
Van Zyl, W.A. (Willem Andries)
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|
dc.contributor.author |
Stutzer, Christian
|
|
dc.date.accessioned |
2013-07-08T08:01:13Z |
|
dc.date.available |
2013-07-08T08:01:13Z |
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dc.date.issued |
2012-06 |
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dc.description.abstract |
In the post-genomic era, reverse vaccinology is proving promising in the development of vaccines against bacterial and viral diseases, with limited application in ectoparasite vaccine design. In this study, we present a systematic approach using a combination of functional genomics (DNA microarrays) techniques and a pipeline incorporating in silico prediction of subcellular localization and protective antigenicity using VaxiJen for the identification of novel anti-tick vaccine candidates. A total of 791 candidates were identified using this approach, of which 176 are membrane-associated and 86 secreted soluble proteins. A preliminary analysis on the antigenicity of selected membrane proteins using anti-gut antisera yielded candidates with an IgG binding capacity greater than previously identified epitopes of Bm86. Subsequent vaccination trials using recombinant proteins will not only validate this approach, but will also improve subsequent reverse vaccinology approaches for the identification of novel anti-tick vaccine candidates. |
en_US |
dc.description.librarian |
hb2013 |
en_US |
dc.description.sponsorship |
The Red Meat Research Development Trust, University of Pretoria Research Development Programme, and the Technology and Human Resources for Industry Programme. |
en_US |
dc.description.uri |
http://www.elsevier.de/ttbdis |
en_US |
dc.identifier.citation |
Maritz-Olivier, C, Van Zyl, W & Stutzer, C 2013, 'A systematic, functional genomics, and reverse vaccinology approach to the identification of vaccine candidates in the cattle tick, Rhipicephalus', Ticks and Tick-borne Diseases, vol. 3, no. 3, pp. 179-187. |
en_US |
dc.identifier.issn |
1877-959X (print) |
|
dc.identifier.issn |
1877-9603 (online) |
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dc.identifier.other |
10.1016/j.ttbdis.2012.01.003 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/21874 |
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dc.language.iso |
en |
en_US |
dc.publisher |
Elsevier |
en_US |
dc.rights |
© 2012 Elsevier GmbH. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Ticks and Tick-borne Diseases. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Ticks and Tick-borne Diseases, vol. 3, no. 3, 2013. DOI : 10.1016/j.ttbdis.2012.01.003 |
en_US |
dc.subject |
Rhipicephalus microplus |
en_US |
dc.subject |
DNA microarray |
en_US |
dc.subject |
Cattle tick |
en_US |
dc.subject |
Reverse vaccinology |
en_US |
dc.subject |
Vaccine discovery |
en_US |
dc.title |
A systematic, functional genomics, and reverse vaccinology approach to the identification of vaccine candidates in the cattle tick, Rhipicephalus |
en_US |
dc.type |
Postprint Article |
en_US |