dc.contributor.author |
Matjila, Mushi
|
|
dc.contributor.author |
Millar, Robert P.
|
|
dc.contributor.author |
Van der Spuy, Zephne
|
|
dc.contributor.author |
Katz, Arieh A.
|
|
dc.date.accessioned |
2013-07-08T07:54:31Z |
|
dc.date.available |
2013-07-08T07:54:31Z |
|
dc.date.issued |
2013-05-16 |
|
dc.description.abstract |
Genes involved in invasion of trophoblast cells and angiogenesis are crucial in determining pregnancy outcome. We
therefore studied expression profiles of these genes in both fetal and maternal tissues to enhance our understanding of
feto-maternal dialogue. We investigated the expression of genes involved in trophoblast invasion, namely Kiss1, Kiss1
Receptor (Kiss1R) and MMP9 as well as the expression of angiogenic ligands Vascular Endothelial Growth Factor-A (VEGF-A)
and Prokineticin-1 (PROK1) and their respective receptors (VEGFR1, VEGFR2 and PROK1R) across the feto-maternal interface
of healthy human pregnancies. The placenta, placental bed and decidua parietalis were sampled at elective caesarean
delivery. Real-time RT-PCR was used to investigate transcription, while immunohistochemistry and western blot analyses
were utilized to study protein expression. We found that the expression of Kiss1 (p,0.001), Kiss1R (p,0.05) and MMP9
(p,0.01) were higher in the placenta compared to the placental bed and decidua parietalis. In contrast, the expression of
VEGF-A was highest in the placental bed (p,0.001). While VEGFR1 expression was highest in the placenta (p,0.01), the
expression of VEGFR2 was highest in the placental bed (p,0.001). Lastly, both PROK1 (p,0.001) and its receptor PROK1R
(p,0.001) had highest expression in the placenta. Genes associated with trophoblast invasion were highly expressed in the
placenta which could suggest that the influence on invasion capacity may largely be exercised at the fetal level.
Furthermore, our findings on angiogenic gene expression profiles suggest that angiogenesis may be regulated by two
distinct pathways with the PROK1/PROK1R system specifically mediating angiogenesis in the fetus and VEGFA/VEGFR2
ligand-receptor pair predominantly mediating maternal angiogenesis. |
en_US |
dc.description.librarian |
am2013 |
en_US |
dc.description.sponsorship |
This work was supported by Tshukululu Trust (Discovery Foundation Academic Fellowship Award) and Netcare’s Physician Trust (Hamilton Naki
Scholarship)who funded the time required for benchwork training of MM. The MRC (SA), Harry Crossley Clinical Research Scholarship, the Nina Lipshitz Fund and
UCT’s Emerging Researcher Project who funded various aspects of the study. |
en_US |
dc.description.uri |
http://www.plosone.org |
en_US |
dc.identifier.citation |
Matjila M, Millar R, van der Spuy Z, Katz A (2013) The Differential Expression of Kiss1, MMP9 and Angiogenic Regulators across the Feto-Maternal Interface of Healthy Human Pregnancies: Implications for Trophoblast Invasion and Vessel Development. PLoS ONE 8(5): e63574. DOI: 10.1371/journal.-pone.0063574 |
en_US |
dc.identifier.issn |
1932-6203 |
|
dc.identifier.other |
10.1371/journal.-pone.0063574 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/21864 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Public Library of Science |
en_US |
dc.rights |
© 2013 Matjila et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License |
en_US |
dc.subject |
Human pregnancies |
en_US |
dc.subject |
Trophoblast invasion |
en_US |
dc.subject |
Vascular Endothelial Growth Factor-A (VEGF-A) |
en_US |
dc.subject |
Prokineticin-1 (PROK1) |
en_US |
dc.subject |
Feto-maternal interface |
en_US |
dc.title |
The differential expression of Kiss1, MMP9 and angiogenic regulators across the feto-maternal interface of healthy human pregnancies : implications for trophoblast invasion and vessel development |
en_US |
dc.type |
Article |
en_US |