dc.contributor.author |
Chapwanya, Aspinas
|
|
dc.contributor.author |
Meade, Kieran G.
|
|
dc.contributor.author |
Doherty, Michael L.
|
|
dc.contributor.author |
Callanan, John J.
|
|
dc.contributor.author |
O’Farrelly, Cliona
|
|
dc.date.accessioned |
2013-06-28T06:47:26Z |
|
dc.date.available |
2013-06-28T06:47:26Z |
|
dc.date.issued |
2013-01 |
|
dc.description.abstract |
Endometrial epithelial cells play a critical role in mediating inflammatory mechanisms key to
bacterial clearance and tissue re-modelling postpartum. This study characterised innate immune
gene expression by bovine endometrial epithelial cells from three animals in response to
Escherichia coli, a common cause of bovine uterine disease. Expression of key innate immune
genes, encoding Toll-like receptor 4 (TLR4), the transcription factor NFkB1, the chemokine
interleukin 8 (IL8), inflammatory cytokines (interleukins IL1β, IL6; tumour necrosis factor,
TNF), β-defensins (lingual antimicrobial peptides LAP, tracheal antimicrobial peptide TAP) and
acute phase proteins (haptoglobin, HP; serum amyloid A, SAA3) was examined in endometrial
epithelial cells stimulated with E. coli for 6 and 24 h using qRT-PCR. Expression of all genes
was increased significantly (P < 0.05) 6 h post-stimulation. Expression of IL1b, TNF and SAA3
genes was increased by 121-, 357- and 721-fold, respectively (P < 0.05). Twenty four hours
post-stimulation, IL1b, IL6, IL8, TNF and LAP gene expression was decreased compared to 6 h,
whereas TAP and SAA3 expression was further increased to 209- and 3452-fold (P < 0.05). E.
coli driven expression of immune effector genes demonstrates potent immune, antimicrobial and
regulatory capacity of endometrial epithelial cells to respond to this pathogen. |
en |
dc.description.librarian |
hb2013 |
en |
dc.description.librarian |
ab2013 |
|
dc.description.uri |
http://www.elsevier.com/locate/vetimm |
en |
dc.identifier.citation |
Chapwanya, A, Meade, KG, Doherty, ML, Callanan, JJ & O’Farrelly, C 2013, 'Endometrial epithelial cells are potent producers of tracheal antimicrobial peptide and serum amyloid A3 gene expression in response to E. coli stimulation', Veterinary Immunology and Immunopathology, vol. 151, no. 1-2, pp. 157-162. |
en |
dc.identifier.issn |
0165-2427 (print) |
|
dc.identifier.issn |
1873-2534 (online) |
|
dc.identifier.other |
10.1016/j.vetimm.2012.09.042 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/21762 |
|
dc.language.iso |
en |
en |
dc.publisher |
Elsevier |
en |
dc.relation.requires |
Adobe Acrobat Reader |
en |
dc.rights |
© 2013 Elsevier. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Veterinary Immunology and Immunopathology.
Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Veterinary Immunology and Immunopathology, vol. 151, no. 1-2, 2013. DOI : 10.1016/j.vetimm.2012.09.042 |
en |
dc.subject |
Acute phase proteins |
en |
dc.subject |
Endometrial epithelial cells |
en |
dc.subject |
Innate immunity |
en |
dc.subject |
Antimicrobial peptide (AMP) |
en |
dc.subject |
Quantitative real time polymerase chain reaction |
en |
dc.subject.lcsh |
Escherichia coli |
en |
dc.subject.lcsh |
Cattle -- Diseases |
en |
dc.title |
Endometrial epithelial cells are potent producers of tracheal antimicrobial peptide and serum amyloid A3 gene expression in response to E. coli stimulation |
en |
dc.type |
Postprint Article |
en |