dc.contributor.author |
Josemans, A.I.
|
|
dc.contributor.editor |
Boomker, Jacob Diederik Frederik |
|
dc.contributor.upauthor |
Zweygarth, Erich
|
|
dc.date.accessioned |
2012-03-06T12:26:33Z |
|
dc.date.available |
2012-03-06T12:26:33Z |
|
dc.date.created |
2012 |
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dc.date.issued |
2001 |
|
dc.description |
The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. |
en |
dc.description.abstract |
The Welgevonden stock of Cowdria ruminantium, aetiologic agent of
heartwater, was continuously propagated in DH82 cells, a continuous
canine macrophage-monocyte cell line. Cultures of DH82 cells were
readily infected provided that the culture medium was supplemented with
cycloheximide. Cultures were split at regular 3-day intervals and
infection rates ranged between 60% and 95%. Cultures were continuously
propagated through more than 125 passages over a period of more than one
year. |
en |
dc.description.librarian |
mn2012 |
en |
dc.description.sponsorship |
Agricultural Research Council of South Africa and the
European Union (Cowdriosis Network) Grant no.
IC18-CT95-0008 (DG12-SNRD). |
en |
dc.identifier.citation |
Zweygarth, E & Josemans, AI 2001, 'Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line’. Onderstepoort Journal of Veterinary Research, vol. 68, no. 2, pp. 155-157. |
en |
dc.identifier.issn |
0030-2465 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/18399 |
|
dc.language |
en |
|
dc.publisher |
Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria. |
en |
dc.rights |
© ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original).
© University of Pretoria. Dept of Library Services (digital). |
en |
dc.subject |
Veterinary medicine |
en |
dc.subject |
Canine macrophage-monocyte cell line |
en |
dc.subject |
Cowdria ruminantium |
en |
dc.subject |
Cycloheximide |
en |
dc.subject |
DH82 |
en |
dc.subject |
Heartwater |
en |
dc.subject |
In vitro cultivation |
en |
dc.subject.lcsh |
Veterinary medicine -- South Africa |
|
dc.subject.lcsh |
Tick-borne diseases in animals |
en |
dc.subject.lcsh |
Heartwater -- South Africa |
en |
dc.subject.lcsh |
Communicable diseases in animals |
en |
dc.title |
Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line |
en |
dc.type |
Article |
en |