The Welgevonden stock of Cowdria ruminantium, aetiologic agent of
heartwater, was continuously propagated in DH82 cells, a continuous
canine macrophage-monocyte cell line. Cultures of DH82 cells were
readily infected provided that the culture medium was supplemented with
cycloheximide. Cultures were split at regular 3-day intervals and
infection rates ranged between 60% and 95%. Cultures were continuously
propagated through more than 125 passages over a period of more than one
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