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1970 Onderstepoort Journal of Veterinary Research, Volume 37, 1970: Recent submissions

  • Failure to demonstrate in vitro as opposed to in vivo transcription of the bluetongue virus genome 
    Verwoerd, Daniel Wynand; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
  • Intersexuality in the horse 
    Gerneke, W.H.; Coubrough, R.I.; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    A cytogenetic and clinical study of an intersexual Arabian horse revealed it to be a genetic female but a gonadal male intersex. This corresponds in general to previously reported cases, which have been genetic females ...
  • Immune response of rabbits, mice and sheep to polyvalent Pasteurella vaccine 
    Cameron, Colin McKenzie; Smit, Gertruida; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    A polyvalent alum-precipitated vaccine containing Pasteurella multocida (Lehmann & Neuman, 1899) types A and D and four serotypes of Pasteurella haemolytica (Newsom & Cross, 1932) produced a sound immunity in mice and sheep ...
  • Relationship of Corynebacterium pseudotuberculosis protoplasmic toxins to the exotoxin 
    Cameron, Colin McKenzie; Smit, Maria C.; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    Corynebacterium pseudotuberculosis (Buchanan, 1911) protoplasm was separated into two toxic fractions by means of gel chromatography. Toxin neutralization tests indicated that one of the fractions is probably identical ...
  • Macromolecular synthesis in bluetongue virus infected cells. II. Host cell metabolism 
    Huismans, H. (Henk), 1942-; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    Infection of L-cells with bluetongue virus results in inhibition of protein and deoxyribonucleic acid synthesis shortly after infection. No inhibition of ribonucleic acid synthesis is observed before 7 hours after infection. ...
  • Macromolecular synthesis in bluetongue virus infected cells. I. Virus-specific ribonucleic acid synthesis 
    Huismans, H. (Henk), 1942-; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    Both virus-specific double-stranded and single-stranded ribonucleic acid (RNA) are synthesized during infection. The single-stranded RNA is formed in a large excess of double-stranded RNA and the rate of synthesis is maximal ...
  • Further observations on the ultrastructure of ephemeral fever virus 
    Lecatsas, G.; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    Electron microscope observations on ephemeral fever virus showed that the electron-dense material was helical in structure, that probably two strands 4 nm in diameter formed a limb approx. 17 nm in diameter with one complete ...
  • Observations on the pathogenesis of bovine and antelope strains of Besnoitia besnoiti (Marotel, 1912) infection in cattle and rabbits 
    Basson, P.A.; McCully, R.M.; Bigalke, R.D.; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    Histopathological studies were made on cattle and rabbits, artificially infected with either proliferative organisms of low and high passage level bovine strains of Besnoitia besnoiti (Marotel, 1912) grown in tissue culture ...
  • A comparative study on the efficiency of water soluble phosphates and a dry phosphate lick for sheep fed on mature grass hay 
    Myburgh, S.J.; Du Toit, J. de V.; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    In a study with adolescent Merino sheep on a diet of mature grass hay, the supplementation with phosphates alone, either in the drinking water or as a dry lick, did not prevent loss of condition. Furthermore, in metabolism ...
  • Relationship of Corynebacterium pseudotuberculosis protoplasmic toxins to the exotoxin 
    Cameron, Colin McKenzie; Smit, Maria C.; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970-06)
    Corynebacterium pseudotuberculosis (Buchanan, 1911) protoplasm was separated into two toxic fractions by means of gel chromatography. Toxin neutralization tests indicated that one of the fractions is probably identical to ...
  • Plaque formation by African horsesickness virus and characterization of its RNA 
    Oellermann, R.A.; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    A method of plaque assay for African horsesickness virus (AHSV) is described. Three cell lines were tested of which BHK21 was the most satisfactory. With an increase in Actinomycin D concentration, an increased inhibition ...
  • Eimeria chinchillae De Vos & Van der Westhuizen, 1968 and other Eimeria spp. from three South African rodent species 
    De Vos, A.J.; Dobson, Lynne D.; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    Eimeria chinchillae De Vos & Van der Westhuizen, 1968, an organism originally described from the chinchilla and subsequently shown to be transmissible to other rodents, was found to occur naturally in Rhabdomys pumilio ...
  • Pathogenesis of heartwater. I. Cowdria ruminantium in the lymph nodes of domestic ruminants 
    Du Plessis, J.L.; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    The intracellular replication of Cowdria ruminantium (Cowdry, 1926) is described in reticulum cells of lymph nodes several days prior to the appearance of rickettsial colonies in vascular endothelial cells. This is ...
  • Experimental infection of game animals with lumpy skin disease virus (prototype strain Neethling) 
    Young, E.; Basson, P.A.; Weiss, K.E.; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    Calves of a giraffe, an impala, two buffaloes, and two adult black wildebeests were artificially infected with the Neethling type of lumpy skin disease virus. The giraffe and impala died with typical symptoms and lesions ...
  • The application of improved techniques to the identification of strains of bluetongue virus 
    Howell, P.G.; Kumm, N.A.; Botha, M.J.; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    Previously the isolation and identification of strains of bluetongue virus has been tedious, so that the introduction of techniques to shorten the delay was considered highly desirable. Neutralization tests were conducted ...
  • Electron microscopy of Cowdria (Rickettsia) ruminantium (Cowdry, 1926) in the endothelial cells of the vertebrate host 
    Pienaar, J.G.; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    The ultrastructure of Cowdria ruminantium was studied in thin sections of choroid plexus from experimentally infected sheep. Glutaraldehyde fixation and osmium tetroxide postfixation were used. The organism developed ...
  • A clinico-pathological study of bilharziasis in sheep 
    Malherbe, W.D.; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    Six healthy sheep were artificially infested through skin exposure with graded doses of cercariae of Schistosoma mattheei Veglia & LeRoux, 1929. By means of laboratory tests the development of lesions due to the disease ...
  • Cytauxzoonosis in a giraffe (Giraffa camelopardalis (Linnaeus, 1758)) in Zululand 
    McCully, R.M.; Keep, M.E.; Basson, P.A.; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    Cytauxzoonosis is reported for the first time in a giraffe [Giraffa camelopardalis (Linnaeus, 1758)] that died naturally of the disease. Both histiotropic and erythrocytic parasites were found. The animal was very anaemic ...
  • Parasitic and other diseases of the African buffalo in the Kruger National Park 
    Basson, P.A.; McCully, R.M.; Kruger, S.P.; Van Niekerk, J.W.; Young, E.; De Vos, Valerius; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    One hundred randomly-collected buffaloes [Syncerus caffer (Sparrman, 1779)] were autopsied, and tissues and parasites were collected for histopathological studies and identification. Blood smears were prepared from 92 ...
  • Studies on the host range of Eimeria chinchillae De Vos & Van der Westhuizen, 1968 
    De Vos, A.J.; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)
    A case of an Eimeria sp. with a lack of host specificity is reported. Eimeria chinchillae DeVos & Van der Westhuizen, 1968, originally described from the chinchilla, was successfully transmitted to seven other rodents, ...