dc.contributor.author |
Peña, Santiago Jr.
|
|
dc.contributor.author |
Gummow, Bruce
|
|
dc.contributor.author |
Parker, Anthony J.
|
|
dc.contributor.author |
Paris, Damien B.B.P.
|
|
dc.date.accessioned |
2024-03-14T10:04:31Z |
|
dc.date.available |
2024-03-14T10:04:31Z |
|
dc.date.issued |
2022-07 |
|
dc.description.abstract |
Despite its potential benefits, the application of frozen-thawed boar spermatozoa in commercial pig production is still very limited. Cryopreservation could induce sperm DNA damage causing reduced fertility and early embryo loss. Glycerol is a popular cryoprotectant used to improve motility and plasma membrane integrity during sperm freezing but the optimal concentration needed to prevent DNA damage without causing cell toxicity is unknown. Thus, the aim of this study was to determine the cryoprotective effect of different concentrations of glycerol on DNA integrity and motility of frozen-thawed boar spermatozoa. Using the TUNEL assay and flow cytometry, there was no significant difference in the percentage of sperm DNA damage between fresh or frozen-thawed sperm at 3%, 6%, and 8% glycerol (1.9 ± 0.4 vs. 3.5 ± 0.8 vs. 2.8 ± 0.5 vs. 3.0 ± 0.8% respectively; P > 0.05). While total and progressively motile spermatozoa were higher in fresh samples, other CASA motion parameters such as straight-line velocity, average path velocity, straightness, and linearity were generally higher in 3% and 6% glycerol-frozen than fresh samples (P ≤ 0.05). At the concentrations tested, glycerol appears to protect DNA integrity of boar spermatozoa during cryopreservation while slightly enhancing some sperm motility parameters. |
en_US |
dc.description.department |
Production Animal Studies |
en_US |
dc.description.librarian |
hj2024 |
en_US |
dc.description.sdg |
None |
en_US |
dc.description.sponsorship |
A JCU Development Grant, College of Public Health, Medical and
Veterinary Sciences PhD Research Funds and the Australia Awards
Scholarship. |
en_US |
dc.description.uri |
https://pjvm-ph.org |
en_US |
dc.identifier.citation |
Peña, S.T., Gummow, B., Parker, A.J. & Paris, D.B.B.P. 2022, 'Boar spermatozoa maintains DNA integrity after cryopreservation using different concentrations of glycerol', Philippine Journal of Veterinary Medicine, vol. 59, no. 2, pp. 164-173. |
en_US |
dc.identifier.issn |
0031-7705 (print) |
|
dc.identifier.issn |
2984-763X (online) |
|
dc.identifier.uri |
http://hdl.handle.net/2263/95208 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
College of Veterinary Medicine, University of the Philippines Los Baños |
en_US |
dc.rights |
© 2022 The Author(s). Published by UP CVM. This is an open access article under the CC BY 4.0 license. |
en_US |
dc.subject |
Boar |
en_US |
dc.subject |
Pigs |
en_US |
dc.subject |
Sperm |
en_US |
dc.subject |
DNA damage |
en_US |
dc.subject |
Deoxyribonucleic acid (DNA) |
en_US |
dc.subject |
Glycerol |
en_US |
dc.subject |
Sperm motility |
en_US |
dc.subject |
Cryopreservation |
en_US |
dc.subject |
Freezing |
en_US |
dc.subject |
TUNEL |
en_US |
dc.subject |
CASA |
en_US |
dc.title |
Boar spermatozoa maintains DNA integrity after cryopreservation using different concentrations of glycerol |
en_US |
dc.type |
Article |
en_US |