Cryopreservation of third-stage larvae of Strongylus vulgaris (large strongyle of horses)

Abstract

A technique for the cryopreservation of third-stage larvae of Strongylus vulgaris is described. Infective larvae of S. vulgaris were exsheathed in a 0,16% sodium hypochlorite solution and then transferred into cryotubes containing 0,09% saline. The samples were stored in the gas phase of liquid nitrogen.