Additional file 1: Sequences of primers used in the study.
Additional file 2: Scripts used for Nesoni, tophat, cufflinks and cuffdiff.
Additional file 3: Figure S2. Schematic representation of the PaLAR3A
promoter (Genbank accession no. KX574229.1) in black and the PaLAR3B
promoter (KX574230.1) in red, the white regions in PaLAR3B promoter
corresponds to deletions in the sequence compared to PaLAR3A promoter.
The two NAC binding sites (TTTCGT) present in the region unique to the
PaLAR3A promoter are indicated in yellow. In the PaLAR3A_mut promoter it
is only these two sites which has been mutated in the remainder of the
PaLAR3A promoter is intact.
Additional file 4: Clustal W Alignment of Norway spruce subgroup III-3
NAC proteins. The coloured boxes correspond to the conserved N-terminal
motifs A (light blue), B (pale green), C (pale red, D (lilac) and E (pale gold).
The shaded residues indicate residues conserved in the C-terminal region.
Additional file 5: Amino acid identity and similarity in subgroup III-3 NAC
proteins. Percent amino acid identity (above the diagonal) and similarity
(below the diagonal) in the complete protein sequences (A) or the C-terminal
part of the proteins (B).
Additional file 6: Relative expression of putative PaNAC3 overexpression
lines. The relative expression was determined in relation to the
untransformed wild type line 95:61:21.
Additional file 7: Transcriptional regulation of PaNAC3 in response to
standard maturation treatment in the wild type line 95:61:21.
Additional file 8: Table S7. RNAseq metrics after Nesoni filtering.
Additional file 9: Alignment summaries from tophat.
Additional file 10: Enriched GO terms among consistently down- or
up-regulated genes in PaNAC3 overexpression lines.
Additional file 11: Consistently up-regulated genes in PaNAC3 overexpression
lines.
Additional file 12: Consistently down-regulated genes in PaNAC3 overexpression
lines.