dc.contributor.author |
De Castro, Minique H.
|
|
dc.contributor.author |
De Klerk, Daniel G.
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|
dc.contributor.author |
Pienaar, Ronel
|
|
dc.contributor.author |
Latif, Abdalla A.
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|
dc.contributor.author |
Rees, D. Jasper G.
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|
dc.contributor.author |
Mans, Ben J. (Barend Johannes)
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|
dc.date.accessioned |
2016-09-22T07:35:31Z |
|
dc.date.issued |
2016-06 |
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dc.description.abstract |
Tick secretory proteins modulate haemostasis, inflammation and immune responses of the host and are attractive recombinant anti-tick vaccine candidates. Yet, many of the proteins have not been characterised due to the limited sequence availability for ticks and other arthropods for homology-based annotation. To address this limitation, we sequenced the salivary glands of the economically important adult male and female Rhipicephalus appendiculatus ticks during feeding. The quality-filtered Illumina sequencing reads were de novo assembled to generate a R. appendiculatus sialotranscriptome of 21,410 transcripts. A non-redundant set of 12,761 R. appendiculatus proteins was predicted from the transcripts, including 2134 putative secretory and 8237 putative housekeeping proteins. Secretory proteins accounted for most of the expression in the salivary gland transcriptome (63%). Of the secretory protein class, the Glycine-rich superfamily contributed 66% and the Lipocalin family 12% of the transcriptome expression. Differential expression analysis identified 1758 female and 2346 male up-regulated transcripts, suggesting varying blood-feeding mechanisms employed between female and male ticks. The sialotranscriptome assembled in this work, greatly improves on the sequence information available for R. appendiculatus and is a valuable resource for potential future vaccine candidate selection. |
en_ZA |
dc.description.department |
Veterinary Tropical Diseases |
en_ZA |
dc.description.embargo |
2017-06-30 |
|
dc.description.librarian |
hb2016 |
en_ZA |
dc.description.sponsorship |
The Economic Competitive Support Programme (30/01/V010) and Incentive Funding for Rated Researchers (NRF-Mans). |
en_ZA |
dc.description.uri |
http://www.elsevier.com/locate/ttbdis |
en_ZA |
dc.identifier.citation |
De Castro, MH, De Klerk, D, Pienaar, R, Latif, AA, Rees, DJG & Mans, BJ 2016, 'De novo assembly and annotation of the salivary gland transcriptome of Rhipicephalus appendiculatus male and female ticks during blood feeding', Ticks and Tick-borne Diseases, vol. 7, no. 4, pp. 536-548. |
en_ZA |
dc.identifier.issn |
1877-959X ( print) |
|
dc.identifier.issn |
1877-9603 (online) |
|
dc.identifier.other |
/10.1016/j.ttbdis.2016.01.014 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/56789 |
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dc.language.iso |
en |
en_ZA |
dc.publisher |
Elsevier |
en_ZA |
dc.rights |
© 2016 Elsevier GmbH. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Ticks and Tick-borne Diseases. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Ticks and Tick-borne Diseases, vol. 7, no. 4, pp. 536-548, 2016. doi : 10.1016/j.ttbdis.2016.01.014. |
en_ZA |
dc.subject |
Rhipicephalus appendiculatus |
en_ZA |
dc.subject |
De novo transcriptome assembly |
en_ZA |
dc.subject |
Salivary glands |
en_ZA |
dc.subject |
Sialotranscriptomics |
en_ZA |
dc.subject |
Secretory proteins |
en_ZA |
dc.subject |
Next generation sequencing (NGS) |
en_ZA |
dc.title |
De novo assembly and annotation of the salivary gland transcriptome of Rhipicephalus appendiculatus male and female ticks during blood feeding |
en_ZA |
dc.type |
Postprint Article |
en_ZA |