dc.contributor.author |
De Maayer, Pieter
|
|
dc.contributor.author |
Chan, Wai Yin
|
|
dc.contributor.author |
Martin, Douglas A.J.
|
|
dc.contributor.author |
Blom, Jochen
|
|
dc.contributor.author |
Venter, S.N. (Stephanus Nicolaas)
|
|
dc.contributor.author |
Duffy, Brion
|
|
dc.contributor.author |
Cowan, Don A.
|
|
dc.contributor.author |
Smits, Theo H.M.
|
|
dc.contributor.author |
Coutinho, Teresa A.
|
|
dc.date.accessioned |
2016-05-20T05:34:52Z |
|
dc.date.available |
2016-05-20T05:34:52Z |
|
dc.date.issued |
2015-06-08 |
|
dc.description.abstract |
Pantoea ananatis is a highly versatile enterobacterium isolated from diverse environmental sources. The ecological diversity of this species may be attributed, in part, to the acquisition of mobile genetic elements. One such element is an Integrative and Conjugative Element (ICE). By means of in silico analyses the ICE elements belonging to a novel family, ICEPan, were identified in the genome sequences of five P. ananatis strains and characterized. PCR screening showed that ICEPan is prevalent among P. ananatis strains isolated from different environmental sources and geographic locations. Members of the ICEPan family share a common origin with ICEs of other enterobacteria, as well as conjugative plasmids of Erwinia spp. Aside from core modules for ICEPan integration, maintenance and dissemination, the ICEPan contain extensive non-conserved islands coding for proteins that may contribute toward various phenotypes such as stress response and antibiosis, and the highly diverse ICEPan thus plays a major role in the diversification of P. ananatis. An island is furthermore integrated within an ICEPan DNA repair-encoding locus umuDC and we postulate its role in stress-induced dissemination and/or expression of the genes on this island. |
en_ZA |
dc.description.department |
Forestry and Agricultural Biotechnology Institute (FABI) |
en_ZA |
dc.description.department |
Genetics |
en_ZA |
dc.description.department |
Microbiology and Plant Pathology |
en_ZA |
dc.description.librarian |
am2016 |
en_ZA |
dc.description.sponsorship |
This study was partially supported by the University of Pretoria Postdoctoral Fellowship Fund, National Research Foundation (NRF), the Tree Protection Co-operative Programme (TPCP), the NRF/Dept. of Science and Technology Centre of Excellence in Tree Health Biotechnology (CTHB), and the THRIP support program of the Department of Trade and Industry, South Africa, the department of Life Sciences and Facility Management of ZHAW, the Swiss Federal Office for Agriculture (BLW Fire Blight Research – Achilles), and the Swiss Secretariat for Education and Research (SBF C07.0038). |
en_ZA |
dc.description.uri |
http://www.frontiersin.org/Microbiology |
en_ZA |
dc.identifier.citation |
De Maayer P, Chan W-Y, Martin DAJ,
Blom J, Venter SN, Duffy B,Cowan
DA, Smits THM and Coutinho TA
(2015) Integrative conjugative
elements of the ICEPan family play a
potential role in Pantoea ananatis
ecological diversification and
antibiosis. Front. Microbiol. 6:576.
DOI: 10.3389/fmicb.2015.00576. |
en_ZA |
dc.identifier.issn |
1664-302X |
|
dc.identifier.other |
10.3389/fmicb.2015.00576 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/52721 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Frontiers Research Foundation |
en_ZA |
dc.rights |
© 2015 Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). |
en_ZA |
dc.subject |
Pantoea ananatis |
en_ZA |
dc.subject |
ICEPan |
en_ZA |
dc.subject |
Stress response |
en_ZA |
dc.subject |
Antibiosis |
en_ZA |
dc.subject |
UmuDC |
en_ZA |
dc.subject |
Integrative and conjugative element (ICE) |
en_ZA |
dc.title |
Integrative conjugative elements of the ICEPan family play a potential role in Pantoea ananatis ecological diversification and antibiosis |
en_ZA |
dc.type |
Article |
en_ZA |