dc.contributor.author |
Chawana, R.
|
|
dc.contributor.author |
Alagaili, Abdulaziz N.
|
|
dc.contributor.author |
Patzke, Nina
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|
dc.contributor.author |
Mohammed, Osama B.
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|
dc.contributor.author |
Kaswera, Consolate
|
|
dc.contributor.author |
Gilissen, Emmanuel
|
|
dc.contributor.author |
Bennett, Nigel Charles
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|
dc.contributor.author |
Ihunwo, Amadi O.
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|
dc.contributor.author |
Manger, Paul R.
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|
dc.contributor.author |
Spocter, M.A.
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|
dc.date.accessioned |
2015-11-18T05:46:25Z |
|
dc.date.available |
2015-11-18T05:46:25Z |
|
dc.date.issued |
2014-09 |
|
dc.description.abstract |
A previous study investigating potential adult hippocampal neurogenesis in microchiropteran bats failed to reveal a strong presence of this neural trait. As microchiropterans have a high field metabolic rate and a small body mass, it is possible that capture/handling stress may lead to a decrease in the detectable presence of adult hippocampal neurogenesis. Here we looked for evidence of adult hippocampal neurogenesis using immunohistochemical techniques for the endogenous marker doublecortin (DCX) in 10 species of microchiropterans euthanized and perfusion fixed at specific time points following capture. Our results reveal that when euthanized and perfused within 15 min of capture, abundant putative adult hippocampal neurogenesis could be detected using DCX immunohistochemistry. Between 15 and 30 min post-capture, the detectable levels of DCX dropped dramatically and after 30 min post-capture, immunohistochemistry for DCX could not reveal any significant evidence of putative adult hippocampal neurogenesis. Thus, as with all other mammals studied to date apart from cetaceans, bats, including both microchiropterans and megachiropterans, appear to exhibit substantial levels of adult hippocampal neurogenesis. The present study underscores the concept that, as with laboratory experiments, studies conducted on wild-caught animals need to be cognizant of the fact that acute stress (capture/handling) may induce major changes in the appearance of specific neural traits |
en_ZA |
dc.description.librarian |
hb2015 |
en_ZA |
dc.description.sponsorship |
South African National Research Foundation (PRM), the Swiss-South African Joint Research Program (AOI and PRM), the Deanship of Scientific Research at the King Saud University through the research group project number RGP_VPP_020 (A.A.), the Belgian co-operation service (DGD) at the Royal Museum for Central Africa (EG), and by a fellowship within the Postdoctoral-Program of the German Academic Exchange Service, DAAD (NP). |
en_ZA |
dc.description.uri |
http://www.journals.elsevier.com/neuroscience |
en_ZA |
dc.identifier.citation |
Chawana, R, Alagaili, AA, Patzke, N, Spocter, MA, Mohammed, OB, Kaswera, C, Gilissen, E, Bennett, NC, Ihunwo, AO & Manger, PR 2014, 'Microbats appear to have adult hippocampal neurogenesis, but post-capture stress causes a rapid decline in the number of neurons expressing doublecortin', Neuroscience, vol. 277, pp. 724-733. |
en_ZA |
dc.identifier.issn |
0306-4522 (print) |
|
dc.identifier.issn |
1873-7544 (online) |
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dc.identifier.other |
10.1016/j.neuroscience.2014.07.063 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/50496 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Elsevier |
en_ZA |
dc.rights |
© 2014 IBRO. Published by Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Neuroscience. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Neuroscience, vol. 277, pp. 724-733, 2014, doi : 10.1016/j.neuroscience.2014.07.063. |
en_ZA |
dc.subject |
Chiroptera |
en_ZA |
dc.subject |
Adult neurogenesis |
en_ZA |
dc.subject |
Capture stress |
en_ZA |
dc.subject |
Doublecortin |
en_ZA |
dc.subject |
Free-living animals |
en_ZA |
dc.subject |
Hippocampus |
en_ZA |
dc.subject |
Bovine serum albumin (BSA) |
en_ZA |
dc.subject |
Diaminobenzidine (DAB) |
en_ZA |
dc.subject |
Doublecortin (DCX) |
en_ZA |
dc.subject |
Doublecortin immunopositive (DCX+) |
en_ZA |
dc.subject |
Granular cell layer (GCL) |
en_ZA |
dc.subject |
Normal rabbit serum (NRS) |
en_ZA |
dc.subject |
Phosphate buffer (PB) |
en_ZA |
dc.subject |
Subventricular zone (SVZ) |
en_ZA |
dc.title |
Microbats appear to have adult hippocampal neurogenesis, but post-capture stress causes a rapid decline in the number of neurons expressing doublecortin |
en_ZA |
dc.type |
Postprint Article |
en_ZA |