dc.contributor.author |
Maharaj, Karishma
|
|
dc.contributor.author |
Bradfield, M.F.A. (Michael Ford Alexander)
|
|
dc.contributor.author |
Nicol, Willie
|
|
dc.date.accessioned |
2015-08-17T12:53:29Z |
|
dc.date.available |
2015-08-17T12:53:29Z |
|
dc.date.issued |
2014-09 |
|
dc.description.abstract |
Continuous anaerobic fermentations were performed
in a biofilm reactor packed with Poraver® beads.
Dilution rates (D) varied between 0.054 and 0.72 h−1, and
D-glucose and CO2 gas were used as carbon substrates.
Steady-state conditions were shown to be repeatable and
independent of the operational history. Production stability
was achieved over periods exceeding 80 h at values of D
below 0.32 h−1. In these situations, steady-state variation
(expressed as fluctuations in NaOH neutralisation flow
rates) exhibited a standard deviation of less than 5 % while
no indication of biofilm deactivation was detected. The
total biomass amount was found to be independent of the
dilution rate with an average dry concentration of 23.8±
2.9 g L−1 obtained for all runs. This suggests that the
attachment area controls the extent of biofilm accumulation.
Specific succinic acid (SA) productivities, based on the
total biomass amount, exhibited a substantial decrease with
decreasing D. An SA volumetric productivity of
10.8 g L−1 h−1 was obtained at D=0.7 h−1—the highest
value reported to date in Actinobacillus succinogenes fermentations.
SA yields on glucose increased with decreasing
D, with a yield of 0.90±0.01 g g−1 obtained at a D of
0.054 h−1. Production of formic acid approached zero with
decreasing D, while the succinic to acetic acid ratio increased
with decreasing D, resulting in an increasing SA
yield on glucose. |
en_ZA |
dc.description.embargo |
2015-09-30 |
en_ZA |
dc.description.librarian |
hb2015 |
en_ZA |
dc.description.sponsorship |
National Research Foundation (NRF) |
en_ZA |
dc.description.uri |
http://link.springer.com/journal/253 |
en_ZA |
dc.identifier.citation |
Maharaj, K, Bradfield, MFA & Nicol, W 2014, 'Succinic acid-producing biofilms of Actinobacillus succinogenes : reproducibility, stability and productivity', Applied Microbiology and Biotechnology, vol. 98, no.17, pp. 7379-7386. |
en_ZA |
dc.identifier.issn |
0175-7598 (print) |
|
dc.identifier.issn |
1432-0614 (online) |
|
dc.identifier.issn |
10.1007/s00253-014-5779-3 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/49355 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Springer |
en_ZA |
dc.rights |
© Springer-Verlag Berlin Heidelberg 2014. The original publication is available at : http://link.springer.comjournal/253. |
en_ZA |
dc.subject |
Actinobacillus succinogenes |
en_ZA |
dc.subject |
Biofilm reactor |
en_ZA |
dc.subject |
Continuous fermentation |
en_ZA |
dc.subject |
Productivity |
en_ZA |
dc.subject |
Stability |
en_ZA |
dc.subject |
Succinic acid |
en_ZA |
dc.title |
Succinic acid-producing biofilms of Actinobacillus succinogenes : reproducibility, stability and productivity |
en_ZA |
dc.type |
Postprint Article |
en_ZA |