dc.contributor.author |
Njage, Patrick Murigu Kamau
|
|
dc.contributor.author |
Buys, E.M. (Elna Maria)
|
|
dc.date.accessioned |
2015-06-22T13:14:26Z |
|
dc.date.available |
2015-06-22T13:14:26Z |
|
dc.date.issued |
2014-05 |
|
dc.description.abstract |
There are few studies on the presence of extendedspectrum
β-lactamases and AmpC β-lactamases
(ESBL/AmpC) in bacteria that contaminate vegetables.
The role of the production environment in ESBL/
AmpC gene transmission is poorly understood. The
occurrence of ESBL/AmpC in Escherichia coli (n = 46)
from lettuce and irrigation water and the role of irrigation
water in the transmission of resistant E. coli
were studied. The presence of ESBL/AmpC, genetic
similarity and phylogeny were typed using genotypic
and phenotypic techniques. The frequency of
β-lactamase gene transfer was studied in vitro.
ESBLs/AmpC were detected in 35 isolates (76%).
Fourteen isolates (30%) produced both ESBLs/AmpC.
Prevalence was highest in E. coli from lettuce (90%).
Twenty-two isolates (48%) were multi-resistant with
between two and five ESBL/AmpC genes. The major
ESBL determinant was the CTX-M type (34 isolates).
DHA (33% of isolates) were the dominant AmpC β
lactamases. There was a high conjugation efficiency
among the isolates, ranging from 3.5 × 10−2 to
1 × 10−2 ± 1.4 × 10−1 transconjugants per recipient.
Water isolates showed a significantly higher conjugation
frequency than those from lettuce. A high degree
of genetic relatedness between E. coli from irrigation
water and lettuce indicated possible common ancestry
and pathway of transmission. |
en_ZA |
dc.description.librarian |
am2015 |
en_ZA |
dc.description.sponsorship |
We acknowledge the TWAS Fellowship for
Research and Advanced Training, National Research Foundation and
Vice-Chancellor Postdoctoral Fellowship Programme at the University
of Pretoria for financing P. M. K. Njage during the research work.
The sequence analysis facility was funded by the National Research
Foundation of South Africa. |
en_ZA |
dc.description.uri |
http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1751-7915 |
en_ZA |
dc.identifier.citation |
Njage, PMK & Buys EM 2014, 'Pathogenic and commensal Escherichia coli from irrigation water show potential in transmission of extended spectrum and AmpC β-lactamases determinants to isolates from lettuce', Microbial Biotechnology, vol. 8, no. 3, pp. 462-473. |
en_ZA |
dc.identifier.issn |
1751-7915 |
|
dc.identifier.other |
10.1111/(ISSN)1751-7915 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/45653 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Wiley Open Access |
en_ZA |
dc.rights |
© 2014 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. |
en_ZA |
dc.subject |
Bacteria |
en_ZA |
dc.subject |
Vegetables |
en_ZA |
dc.subject |
Escherichia coli |
en_ZA |
dc.subject |
Lettuce |
en_ZA |
dc.title |
Pathogenic and commensal Escherichia coli from irrigation water show potential in transmission of extended spectrum and AmpC β-lactamases determinants to isolates from lettuce |
en_ZA |
dc.type |
Article |
en_ZA |