dc.contributor.author |
Contrafatto, G.
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dc.contributor.author |
Pammenter, M.D.
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dc.contributor.editor |
Bigalke, R.D. |
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dc.date.accessioned |
2014-10-15T10:39:16Z |
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dc.date.available |
2014-10-15T10:39:16Z |
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dc.date.created |
2014 |
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dc.date.issued |
1988 |
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dc.description |
The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. |
en |
dc.description.abstract |
Various immunization procedures were investigated in an effort to improve the number of hybridomas producing antibodies against Cysticercus cellulosae. Ten groups of 5 BALB/c mice were subjected to different immunization procedures and were bled repeatedly over a period of 68 days. The samples of sera thus obtained were tested by enzyme linked immunosorbent assay : total immunoglobulins, lgG and IgM levels were determined. In general, total anticyst antibody titres increased during the course of immunization but in 3 groups the final titre was lower than the maximal antibody titre. Overall, immune tolerance did not appear to be a problem and longer immunization programs seemed to end with slightly higher antibody levels. So far, 4 mice from the group that exhibited the highest immunoglobulin levels have been used for hybridoma production. Out of 124 hybridomas thus obtained, only 1 secreted antibodies against Cysticercus cellulosae. |
en |
dc.description.librarian |
lmchunu2014 |
en |
dc.description.librarian |
mn2014 |
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dc.identifier.citation |
Contrafatto, G & Pammenter, MD 1988, 'Immunization procedure-related immunoglobulin levels in the development of antibodies against Cysticercus cellulosae', Onderstepoort Journal of Veterinary Research, vol. 55, no. 3, pp. 169-172. |
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dc.identifier.issn |
0330-2465 |
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dc.identifier.uri |
http://hdl.handle.net/2263/42380 |
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dc.language.iso |
en |
en |
dc.publisher |
Published by the Government Printer, Pretoria. |
en |
dc.rights |
©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital). |
en |
dc.subject |
Veterinary medicine |
en |
dc.subject.lcsh |
Veterinary medicine -- South Africa |
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dc.title |
Immunization procedure-related immunoglobulin levels in the development of antibodies against Cysticercus cellulosae |
en |
dc.type |
Article |
en |