Browsing 1987 Onderstepoort Journal of Veterinary Research, Volume 54, 1987 by Title

Browsing 1987 Onderstepoort Journal of Veterinary Research, Volume 54, 1987 by Title

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  • The present state of Cowdria ruminantium cultivation in cell lines 
    Bezuidenhout, J.D.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Attempts were made to grow 4 isolates of Cowdria ruminantium in cell lines. Three of these isolates, viz. Ball 3, Welgevonden and Kwanyanga, could be cultivated in a calf endothelial cell line, but experiments with the ...
  • Problems encountered in the control of heartwater in Angora goats 
    Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa); Gruss, B.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    This preliminary investigation confirmed that when Angora goats were immunized against heartwater, either when kids or young goats, they still had an immunity to heartwater a year later when they were challenged with a ...
  • Problems with the interpretation of epidemiological data in heartwater : a study on 23 farms 
    Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa); Malan, Letitia; Du Plessis, J.L.; Bigalke, R.D. (Pretoria : Government Printer, 1987)
    In an epidemiological study undertaken on 23 farms where heartwater occurs endemically, it was found that on an overall average, antibodies to Cowdria ruminantium were detected in 64,3 % of the cattle, 6 adult Amblyomma ...
  • Production of a macrophage chemotactic factor by cultured jaagsiekte tumour cells 
    Verwoerd, Daniel Wynand; Myer, M.S.; Garnett, H.M.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    The increase of alveolar macrophages in jaagsiekte sheep lungs is not caused by excessive surfactant production but is due to a chemotactic factor secreted by the tumour cells. This factor has a molecular mass in the region ...
  • The production of heartwater vaccine 
    Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa); Oberem, P.T.; Bezuidenhout, J. Dürr; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    The events leading to the production of an effective vaccine against heartwater are summarized. The production techniques used at Onderstepoort to produce and control the quality of the infected blood and nymph suspension ...
  • Purification of Cowdria ruminantium by density gradient centrifugation 
    Neitz, Albert Walter Herman; Vermeulen, N.M.J.; Viljoen, G.J.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    The isolation of Cowdria ruminantium by differential and isopycnic density gradient centrifugation is reviewed with special reference to the suitability of Percoll as density gradient medium. Infected sheep brain, Amblyomma ...
  • Purification of Cowdria ruminantium by immunoadsorbent affinity chromatography 
    Neitz, Albert Walter Herman; Vermeulen, N.M.J.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Immunoselective methods with special reference to immunoadsorbent affinity chromatography as a means for the isolation of Cowdria ruminantium are reviewed. Attention is given to the source of the organism, immunization, ...
  • Purification of Cowdria ruminantium by lectin cellular affinity chromatography 
    Vermeulen, N.M.J.; Neitz, Albert Walter Herman; Viljoen, G.J.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    This review covers the isolation of Cowdria ruminantium by lectin cellular affinity chromatography from different Amblyomma hebraeum sources. Cellular affinity chromatography has been reviewed with special attention being ...
  • Purification of the alpha toxin of Clostridium perfringens type A by ultrafiltration and gel chromatography 
    Odendaal, M.W.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Clostridium perfringens type A toxin produced in Jayko & Lichstein medium was subjected to various concentration and purification procedures. The results obtained with 3 different ultrafiltration membranes followed by gel ...
  • A rapid microtitration serum agglutination test for the detection of contagious equine metritis antibodies 
    Gummow, Bruce; Herr, S.; Brett, O.L.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    A microtitration serum agglutination test, based on that used for brucellosis, has been developed to detect antibodies in the sera of horses exposed to the contagious equine metritis (CEM) organism. Two known positive sera ...
  • Rearing and infection techniques for Amblyomma species to be used in heartwater transmission experiments 
    Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa); Heyne, Heloise; Elliott, E.G.R.; Bezuidenhout, J. Dürr; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Techniques for the rearing of Amblyomma species to be used in heartwater (Cowdria ruminantium) transmission experiments are discussed. These involve the breeding and maintenance of infected and noninfected strains of ticks. ...
  • The relationship between the skin and some bacterial species occurring on it in the Merino 
    Jansen, B.C.; Hayes, M; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Ten bacterial species were isolated from areas on the skin of Merino sheep affected by a chronic scaly condition leading to retarded wool growth. The growth requirements of the bacteria were studied and it was shown that ...
  • The reservoir status of goats recovered from heartwater 
    Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa); Barre, N.; Camus, E.; Bigalke, R.D. (Pretoria : Government Printer, 1987)
    Experiments were conducted with Creole goats and Amblyomma variegatum ticks in Guadeloupe to investigate whether it is possible to transmit Cowdria ruminantium to susceptible hosts with nymphs fed in the larval stage on ...
  • Resistance by the blue tick (Boophilus decoloratus) to the synthetic pyrethroid, fenvalerate 
    Coetzee, Bridgette B.; Stanford, G.D.; Davis, D.A.T.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    The Shaw larval test, in conjunction with adult tick immersion and stall tests, was utilized to confirm that a field strain of B. decoloratus, from Natal, is highly resistant to the ixodicide fenvalerate (Factor of ...
  • The resistance spectrum shown by a fenvalerate-resistant strain of blue tick (Boophilus decoloratus) to a range of ixodicides 
    Coetzee, Bridgette B.; Stanford, G.D.; Davis, D.A.T.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    A strain of Boophilus decoloratus, resistant to fenvalerate, was subjected to larval immersion, adult immersion and stall tests using the following classes of ixodicides : organochlorines, organophosphates, a diamidide and ...
  • Resistance to various antibiotics of Salmonella and Escherichia coli isolated from registrable farm feeds 
    Durand, Anette M.; Barnard, Marie-Luise; Swanepoel, Martha L.; Engelbrecht, Marie M.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Resistance to 20 antibiotics of 128 Salmonella and 97 Escherichia coli isolates from various registrable farm feeds was determined. A high frequency of comparatively low levels of resistance was found in both the Salmonella ...
  • Seasonal abundance of carrion-frequenting blow-flies (Diptera : Calliphoridae) in the Kruger National Park 
    Braack, L.E.O.; De Vos, Valerius; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Monthly population fluctuations of carrion-frequenting blow-flies over a 24-month period were monitored using 2 carrion-baited traps in the southern Kruger National Park (KNP) and 3 in the northern KNP. All species displayed ...
  • The serological relationship between Cowdria ruminantium and certain members of the genus Ehrlichia 
    Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa); Holland, C.J.; Logan, L.L.; Mebus, C.A.; Ristic, M.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Although Cowdria ruminantium has been classified within the tribe Ehrlichia of the family Rickettsiaceae, there has been no extensive study conducted regarding its antigenic relationship to members of the genus Ehrlichia, ...
  • A single dilution enzyme-linked immunosorbent assay for the quantitative detection of antibodies to African horsesickness virus 
    Williams, R.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    A single dilution enzyme-linked immunosorbent assay (ELISA), utilizing a reference serum with a known, predetermined end-titre was used to compute antibody titres of serum samples against African horsesickness virus. ...
  • Some avian and mammalian hosts of Amblyomma hebraeum and Amblyomma marmoreum (Acari : Ixodidae) 
    Horak, Ivan Gerard; Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa); MacIvor, K.M. de F.; Petney, T.N.; De Vos, Valerius; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Large numbers of birds, wild mammals and domestic stock from a variety of localities within the Republic of South Africa were examined for infestation with the ixodid ticks Amblyomma hebraeum and Amblyomma marmoreum. Every ...