dc.contributor.author |
Ahmed, Aroke Shahid
|
|
dc.contributor.author |
McGaw, Lyndy Joy
|
|
dc.contributor.author |
Eloff, Jacobus Nicolaas
|
|
dc.date.accessioned |
2014-07-09T07:32:16Z |
|
dc.date.available |
2014-07-09T07:32:16Z |
|
dc.date.issued |
2013-05 |
|
dc.description.abstract |
BACKGROUND: Microbial infections and resulting inflammation and oxidative stress are common pathogenesis of
gastrointestinal tract (GIT) disorders. In South Africa, several species of the genus Maytenus are used in traditional
medicine to treat various infectious diseases. Most of the previous work on this genus was focused on nonpolar
extracts from the root and bark. In this study, leaf extracts of polar extracts of Maytenus peduncularis, Maytenus
procumbens, Maytenus senegalensis and Maytenus undata were evaluated for antimicrobial, anti-inflammatory and
antioxidant activities to determine their efficacy as therapeutic agents in GIT disorders.
METHODS: Phenolic-enriched leaf extracts and fractions were prepared by extracting with acidified 70% methanol
and solvent-solvent fractionation. The activities of the fractions against Staphylococcus aureus, Pseudomonas
aeruginosa, Escherichia coli and Enterococcus faecalis as well as clinical isolates of Aspergillus fumigatus, Candida
albicans and Cryptococcus neoformans were determined using a serial microplate dilution method. Antioxidant
activities were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis(3-ethylbenzthiazoline-6-
sulphonic acid) (ABTS), hydroxyl (OH) radical scavenging and linoleic acid peroxidation inhibitory assays. The
phenolic composition as well as the cytotoxicity against Vero cell lines of the crude extracts was evaluated using
various standard protocols.
RESULTS: The antimicrobial activities were concentrated in the non-polar fractions of hexane, dichloromethane and
ethyl acetate (MICs 19–312 μg/ml). The crude extracts and polar fractions (butanol and water) had moderate to
poor antimicrobial activity (MICs 312 to above 2500 μg/ml). The crude extracts and polar fractions had good
antioxidant activity (EC50 values varied from 1.22 to 607 μg/ml, 1.71 to 312 μg/ml and 23 to 284 μg/ml for DPPH,
ABTS and OH respectively. Linoleic acid peroxidation inhibition EC50 values of the crude extracts ranged between
27 and 39 μg/ml with relatively low toxicity against Vero cell lines (IC50 values 87 to 187 μg/ml). Fractionation of a
crude extract with low activity could lead to fractions with more potent activity. CONCLUSION: This study justifies the traditional use of leaf crude extracts and fractions from these four plants to
remedy gastrointestinal disorders resulting from infection, inflammation and oxidative stress complications. The
study also provides rationale for the use of leaf extracts with same beneficial effects in place of unsustainable root
and bark harvest. |
en_US |
dc.description.librarian |
am2014 |
en_US |
dc.description.sponsorship |
The South African Medical Research Council |
en_US |
dc.description.uri |
http://www.biomedcentral.com/bmccomplementalternmed |
en_US |
dc.identifier.citation |
Ahmed, AS, McGaw, LJ & Eloff, JN 2013, 'Evaluation of pharmacological activities, cytotoxicity and phenolic composition of four Maytenus species used in southern African traditional medicine to treat intestinal infections and diarrhoeal diseases', BMC Complementary and Alternative Medicine, vol. 13, art:100, pp. 1-15. |
en_US |
dc.identifier.issn |
1472-6882 |
|
dc.identifier.other |
10.1186/1472-6882-13-100 |
|
dc.identifier.other |
55952645400 |
|
dc.identifier.other |
7005589445 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/40675 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
BioMed Central |
en_US |
dc.rights |
© 2013 Ahmed et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License |
en_US |
dc.subject |
Maytenus |
en_US |
dc.subject |
Infections |
en_US |
dc.subject |
Diarrhoea |
en_US |
dc.subject |
Cytotoxicity |
en_US |
dc.subject |
Gastrointestinal tract |
en_US |
dc.title |
Evaluation of pharmacological activities, cytotoxicity and phenolic composition of four Maytenus species used in southern African traditional medicine to treat intestinal infections and diarrhoeal diseases |
en_US |
dc.type |
Article |
en_US |