dc.contributor.author |
Kgaladi, Joe
|
|
dc.contributor.author |
Wright, Nicolette
|
|
dc.contributor.author |
Coertse, Jessica
|
|
dc.contributor.author |
Markotter, Wanda
|
|
dc.contributor.author |
Marson, Denise
|
|
dc.contributor.author |
Fooks, Anthony R.
|
|
dc.contributor.author |
Freuling, Conrad M.
|
|
dc.contributor.author |
Muller, Thomas F.
|
|
dc.contributor.author |
Sabeta, Claude Taurai
|
|
dc.contributor.author |
Nel, Louis Hendrik
|
|
dc.contributor.editor |
Rupprecht, Charles Edward |
|
dc.date.accessioned |
2014-05-19T07:46:58Z |
|
dc.date.available |
2014-05-19T07:46:58Z |
|
dc.date.issued |
2013-10-24 |
|
dc.description.abstract |
Mokola virus (MOKV) appears to be exclusive to Africa. Although the first isolates were from Nigeria and other Congo basin
countries, all reports over the past 20 years have been from southern Africa. Previous phylogenetic studies analyzed few
isolates or used partial gene sequence for analysis since limited sequence information is available for MOKV and the isolates
were distributed among various laboratories. The complete nucleoprotein, phosphoprotein, matrix and glycoprotein genes
of 18 MOKV isolates in various laboratories were sequenced either using partial or full genome sequencing using
pyrosequencing and a phylogenetic analysis was undertaken. The results indicated that MOKV isolates from the Republic of
South Africa, Zimbabwe, Central African Republic and Nigeria clustered according to geographic origin irrespective of the
genes used for phylogenetic analysis, similar to that observed with Lagos bat virus. A Bayesian Markov-Chain-Monte-Carlo-
(MCMC) analysis revealed the age of the most recent common ancestor (MRCA) of MOKV to be between 279 and 2034 years
depending on the genes used. Generally, all MOKV isolates showed a similar pattern at the amino acid sites considered
influential for viral properties. |
en_US |
dc.description.librarian |
am2014 |
en_US |
dc.description.sponsorship |
The South African National Research Foundation (NRF) and the South African Polioemylitis Research Foundation (PRF) as well as
grants from the Department for Environment, Food and Rural Affairs (SE0423 and SE0427), the Department of Science and
Technology (04/17/c215), BMBF (01KI1016A), the Research and Policy for Infectious Disease Dynamics (RAPIDD) programme of the Science and Technology
Directorate, US Department of Homeland Security, at the Fogarty International Center, National Institutes of Health, and by the EU FP7–funded Research
Infrastructure Grant ‘‘European Virus Archive’’ (no. 19 228292). |
en_US |
dc.description.uri |
http://www.plosntds.org |
en_US |
dc.identifier.citation |
Kgaladi J, Wright N, Coertse J, Markotter W, Marston D, et al. (2013) Diversity and Epidemiology of Mokola Virus. PLoS Negl Trop Dis 7(10): e2511. DOI: 10.1371/journal.pntd.0002511 |
en_US |
dc.identifier.issn |
1932-6203 |
|
dc.identifier.other |
10.1371/journal.pntd.0002511 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/39811 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Public Library of Science |
en_US |
dc.rights |
© 2013 Kgaladi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License |
en_US |
dc.subject |
Africa |
en_US |
dc.subject |
Southern Africa |
en_US |
dc.subject |
Diversity |
en_US |
dc.subject |
Epidemiology |
en_US |
dc.subject |
Mokola virus (MOKV) |
en_US |
dc.title |
Diversity and epidemiology of Mokola virus |
en_US |
dc.type |
Article |
en_US |