Canine serum amyloid A (SAA) measured by automated latex agglutination turbidimetry is useful for routine sensitive and specific detection of systemic inflammation in a general clinical setting

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dc.contributor.author Christensen, Michelle B.
dc.contributor.author Langhorn, Rebecca
dc.contributor.author Goddard, Amelia
dc.contributor.author Andreasen, Eva B.
dc.contributor.author Moldal, Elena
dc.contributor.author Tvarijonavictute, Asta
dc.contributor.author Kirpenteijn, Jolle
dc.contributor.author Jakobsen, Sabrina
dc.contributor.author Persson, Frida
dc.contributor.author Kjelgaard-Hansen, Mads
dc.date.accessioned 2014-04-10T10:30:46Z
dc.date.available 2014-04-10T10:30:46Z
dc.date.issued 2013-11-28
dc.description.abstract Canine serum amyloid A (SAA) is a useful diagnostic marker of systemic inflammation. A latex agglutination turbidimetric immunoassay (LAT) was validated for automated measurements. The aim of the study was to evaluate the clinical applicability of SAA measured by the LAT. SAA was measured in 7 groups of dogs with and without systemic inflammation (n=247). Overlap performance was investigated. Diagnostic performance was compared to body temperature and leukocyte markers. Clinical decision limits for SAA were estimated. In dogs with neurological, neoplastic or gastrointestinal disorders (n=143), it was investigated whether a higher proportion of SAA positive dogs could be detected in cases of complications with risk of systemic inflammation. Significantly higher concentrations of SAA were measured in dogs with (range [48.75; 5,032 mg/l]), compared to dogs without systemic inflammation [0; 56.4 mg/l]. SAA was a more sensitive and specific marker of systemic inflammation (area under the receiver-operating characteristic curve (AUC) 1.00), compared to body temperature (0.6) and segmented neutrophils (best performing leukocyte marker, 0.84). A clinical decision limit of 56.4 mg/l was established giving close to perfect discrimination between dogs with and without systemic inflammation. Higher proportions of SAApositive dogs were observed in dogs with neurological, neoplastic and gastrointestinal disorders with complications known to increase risk of systemic inflammation, compared to uncomplicated cases. The automated LAT makes SAA applicable as a relevant diagnostic marker of systemic inflammation in dogs for routine random-access real-time use in a general clinical setting. en
dc.description.librarian am2014 en
dc.description.librarian mn2014
dc.description.sponsorship The EIKEN Chemical Co., Tokyo, Japan en
dc.description.uri https://www.jstage.jst.go.jp/browse/jvms en
dc.identifier.citation Christensen, MB, Langhorn, R, Goddard, A, Andreasen, EB, Moldal, E, Tvarijonaviciute, A, Kirpenteijn, J, Jakobsen, S, Persson, F & Kjelgaard-Hansen, M 2013, 'Canine serum amyloid A (SAA) measured by automated latex agglutination turbidimetry is useful for routine sensitive and specific detection of systemic inflammation in a general clinical setting', Journal of Veterinary Medical Science, vol. 75, no. 4, pp. 459-466. en
dc.identifier.issn 0916-7250 (print)
dc.identifier.issn 1347-7439 (online)
dc.identifier.other 10.1292/jvms.12-0404
dc.identifier.uri http://hdl.handle.net/2263/39639
dc.language.iso en en
dc.publisher Maruzen Company Ltd en
dc.rights © 2013 The Japanese Society of Veterinary Science en
dc.subject Veterinary clinical pathology en
dc.subject Canine serum amyloid A (SAA) en
dc.subject Systemic inflammation en
dc.subject Latex agglutination turbidimetric immunoassay (LAT) en
dc.subject Canine en
dc.subject Inflammation en
dc.subject.lcsh Veterinary medicine -- Research en
dc.title Canine serum amyloid A (SAA) measured by automated latex agglutination turbidimetry is useful for routine sensitive and specific detection of systemic inflammation in a general clinical setting en
dc.type Article en


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