Inhaled microparticles containing clofazimine are efficacious in treatment of experimental tuberculosis in mice

Verma, Rahul Kumar; Germishuizen, Willem Andreas; Motheo, M.P. (Mamofalali Portia); Agrawal, Atul Kumar; Singh, Amit Kumar; Mohan, Mradul; Gupta, Pushpa; Gupta, Umesh Datta; Cholo, Moloko C.; Anderson, Ronald; Fouche, P. Bernard; Misra, Amit

dc.contributor.author	Verma, Rahul Kumar
dc.contributor.author	Germishuizen, Willem Andreas
dc.contributor.author	Motheo, M.P. (Mamofalali Portia)
dc.contributor.author	Agrawal, Atul Kumar
dc.contributor.author	Singh, Amit Kumar
dc.contributor.author	Mohan, Mradul
dc.contributor.author	Gupta, Pushpa
dc.contributor.author	Gupta, Umesh Datta
dc.contributor.author	Cholo, Moloko C.
dc.contributor.author	Anderson, Ronald
dc.contributor.author	Fouche, P. Bernard
dc.contributor.author	Misra, Amit
dc.date.accessioned	2014-04-10T09:13:00Z
dc.date.available	2014-04-10T09:13:00Z
dc.date.issued	2013-02
dc.description.abstract	Inhalable clofazimine-containing dry powder microparticles (CFM-DPI) and native clofazimine (CFM) were evaluated for activity against Mycobacterium tuberculosis in human monocyte-derived macrophage cultures and in mice infected with a low-dose aerosol. Both formulations resulted in 99% killing at 2.5 g/ml in vitro. In mice, 480 g and 720 g CFM-DPI inhaled twice per week over 4 weeks reduced numbers of CFU in the lung by as much as log10 2.6; 500 g oral CFM achieved a log10 0.7 reduction.	en_US
dc.description.librarian	am2014	en_US
dc.description.librarian	ay2014
dc.description.sponsorship	The Indian work was funded by a grant from CSIR, India (NWP0035). R.K.V., A.K.S., and A.K.A. received research fellowships from CSIR, India, and M.M. received one from ICMR, India. The South African work was supported by The South African Medical Research Council (M.P.M., M.C., R.A.) and a K-RITH collaborative grant (Howard Hughes Medical Institute and the University of KwaZulu-Natal, to P.B.F. and W.A.G.).	en_US
dc.description.uri	http://aac.asm.org/	en_US
dc.identifier.citation	Verma, RK, Germishuizen, WA, Motheo, MP, Agrawal, AK, Singh, AK, Mohan, M, Gupta, P, Gupta, UD, Cholo, M, Anderson, R, Fourie, PB & Misra, A 2013, 'Inhaled microparticles containing clofazimine are efficacious in treatment of experimental tuberculosis in mice', Antimicrobial Agents and Chemotherapy , vol. 57, no. 2, pp. 1050-1052.	en_US
dc.identifier.issn	0066-4804 (print)
dc.identifier.issn	1098-6596 (online)
dc.identifier.other	10.1128/AAC.01897-12
dc.identifier.uri	http://hdl.handle.net/2263/39634
dc.language.iso	en	en_US
dc.publisher	American Society for Microbiology	en_US
dc.rights	© 2013, American Society for Microbiology. All Rights Reserved.	en_US
dc.subject	Microparticles	en_US
dc.subject	Clofazimine	en_US
dc.subject	Treatment	en_US
dc.subject	Mice	en_US
dc.subject.lcsh	Tuberculosis -- Treatment -- Research	en
dc.title	Inhaled microparticles containing clofazimine are efficacious in treatment of experimental tuberculosis in mice	en_US
dc.type	Article	en_US