Browsing South African National Veterinary Repository by UP Author "Zweygarth, Erich"

Browsing South African National Veterinary Repository by UP Author "Zweygarth, Erich"

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  • A chemically defined medium for the growth of Cowdria ruminantium 
    Zweygarth, Erich; Josemans, A.I.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2001)
    Chemically defined media, termed SFMC-23 and SFMC-36, were devised for the in vitro culture of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants. Both media were based on Dulbecco's ...
  • Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line 
    Zweygarth, Erich; Josemans, A.I.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2001)
    The Welgevonden stock of Cowdria ruminantium, aetiologic agent of heartwater, was continuously propagated in DH82 cells, a continuous canine macrophage-monocyte cell line. Cultures of DH82 cells were readily ...
  • Evaluation of a short-term in vitro growth-inhibition test to determine susceptibility of Trypanosoma vivax stocks to various trypanocides 
    Zweygarth, Erich; Kaminsky, R.; Moloo, S.K.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1994)
    Two Trypanosoma vivax stocks were initiated in culture with tsetse or culture-derived metacyclics. They were propagated axenically as bloodstream trypomastigotes at 35°C in 4% C0₂ in air. Populations of trypanosomes were ...
  • In vitro cultivation of a Babesia sp. from cattle in South Africa 
    Zweygarth, Erich; Van Niekerk, C.; Just, M.C.; De Waal, D.T.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)
    A South African Babesia sp. of cattle which is as yet unclassified, was continuously cultivated in micro-aerophilous stationary-phase culture. The parasites were resuscitated from a blood stabilate stored in liquid nitrogen. ...
  • In vitro cultivation of Babesia equi: detection of carrier animals and isolation of parasites 
    Zweygarth, Erich; Just, M.C.; De Waal, D.T.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1997)
    By means of an in vitro culture technique, 75 samples of horse blood were examined for Babesia equi, a causative agent of equine piroplasmosis. At the time of culture initiation, 15 samples were microscopically positive ...
  • In vitro cultivation of Babesia occultans 
    Van Niekerk, C.J.; Zweygarth, Erich; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1996)
    Babesia occultans, the causative agent of a benign form of cattle babesiosis in South Africa, was continuously cultivated in microaerophilous stationary-phase culture. A modified medium, 199, supplemented with either ...
  • In vitro infection by Ehrlichia ruminantium of baby hamster kidney (BHK), Chinese hamster ovary (CHO-K1) and Madin Darby bovine kidney (MDBK) cells 
    Zweygarth, Erich; Josemans, A.I.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2003)
    The Welgevonden stock of Ehrlichia ruminantium, aetiological agent of heartwater, was propagated in baby hamster kidney (BHK) cells, Chinese hamster ovary (CHO-K1) cells and Madin Darby bovine kidney (MDBK) cells. The ...
  • In vitro isolation of equine piroplasms derived from Cape Mountain zebra (Equus zebra zebra) in South Africa 
    Zweygarth, Erich; Meyer, P.; Lopez-Rebollar, Laura M.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Twenty blood samples of zebras ( Equus zebra zebra) from the Karoo National Park and the Bontebok National Park in South Africa, all seropositive for Theileria equi, were subjected to in vitro culture to identify carrier ...
  • The Kumm isolate of Ehrlichia ruminantium: in vitro isolation, propagation and characterization 
    Zweygarth, Erich; Van Heerden, Henriette; Allsopp, Basil A.; Josemans, A.I.; Van Strijp, M.F.; Allsopp, M.T.E.P.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    An effective culture system for Ehrlichia (Cowdria) ruminantium comb. nov. was first established in 1985 and many stocks were subsequently isolated and propagated in vitro. A notable exception, however, was the Kumm isolate ...