Cryopreservation of sheathed third-stage larvae of Gaigeria pachyscelis (Sandveld hookworm)

Titoy, G.A.P.; Schwan, Ernst Volker

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1995 Onderstepoort Journal of Veterinary Research, Volume 62, 1995
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dc.contributor.author	Titoy, G.A.P.
dc.contributor.author	Schwan, Ernst Volker
dc.contributor.editor	Verwoerd, Daniel Wynand
dc.date.accessioned	2013-09-03T10:37:55Z
dc.date.available	2013-09-03T10:37:55Z
dc.date.created	2013
dc.date.issued	1995
dc.description	The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.	en
dc.description.abstract	A technique for the cryopreservation of third stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed third-stage larvae in 80% (v/v) ethylene glycol as a cryoprotectant for 30 s at 0°C, prior to transfer into liquid nitrogen. The survival rate, as assessed by motility, was 37,6% after 30 d cryopreservation. A sheep infected percutaneously with 700 live cryopreserved third-stage larvae, harboured 41 adult worms (infectivity rate: 5,8%) when necropsied 78 d later.	en
dc.description.librarian	mn2013
dc.identifier.citation	Titoy, GAP & Schwan, EV 1995, 'Cryopreservation of sheathed third-stage larvae of Gaigeria pachyscelis (Sandveld hookworm)’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 1, pp. 63-64.	en
dc.identifier.issn	0330-2465
dc.identifier.uri	http://hdl.handle.net/2263/22262
dc.language.iso	en	en
dc.publisher	Published by the Agricultural Research Council, Onderstepoort Veterinary Institute	en
dc.rights	© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).	en
dc.subject	Veterinary medicine	en
dc.subject	Cryopreservation	en
dc.subject	Sheathed third-stage larvae	en
dc.subject	Gaigeria pachyscelis	en
dc.subject	Sandveld hookworm	en
dc.subject.lcsh	Veterinary medicine -- South Africa
dc.title	Cryopreservation of sheathed third-stage larvae of Gaigeria pachyscelis (Sandveld hookworm)	en
dc.type	Article	en