dc.contributor.author |
Paparu, Pamela
|
|
dc.contributor.author |
Dubois, Thomas
|
|
dc.contributor.author |
Coyne, Daniel
|
|
dc.contributor.author |
Viljoen, Altus
|
|
dc.date.accessioned |
2013-06-04T11:46:16Z |
|
dc.date.available |
2013-06-04T11:46:16Z |
|
dc.date.issued |
2013-04 |
|
dc.description.abstract |
Endophytic non-pathogenic Fusarium oxysporum isolates are known to control Radopholus similis through
induced resistance. Using complementary (c)DNA-amplified fragment length polymorphism (cDNA-AFLP),
we identified genes induced in nematode-susceptible and -tolerant bananas following colonization by nonpathogenic
endophytic F. oxysporum isolate V5w2. Quantitative real-time polymerase chain reactions (qRTPCR)
were then used to analyze expression profiles of selected genes. Accumulated gene transcripts included
those involved in signal transduction, cell wall strengthening, Jasmonic acid (JA) pathway and transport of
defense molecules. Endophyte colonization primed the expression of β-1,3-glucan synthase and Coronatine
insensitive 1 (COI1) in the susceptible cultivar, and that of COI1 and Lipoxygenase (LOX) in the tolerant. We
isolate and identify for the first time genes involved in the interaction between endophytic non-pathogenic
F. oxysporum and banana.
Highlights
· We report identification of genes involved in fungal endophyte protection of banana.
· We report JA-induced defenses in banana- V5w2- Radopholus similis interaction.
· Genes were primed for expression upon nematode challenge of V5w2 inoculated plants. |
en_US |
dc.description.librarian |
hb2013 |
en_US |
dc.description.sponsorship |
We acknowledge the Bundesministerium für Wirtschaftliche Zusammenarbeit (BMZ) for funding this study. |
en_US |
dc.description.uri |
http://www.elsevier.com/locate/pmpp |
en_US |
dc.identifier.citation |
Paparu, P, Dubois, T , Coyne, D & Viljoen, A 2013, 'Differential gene expression in East African highland bananas (Musa spp.) : interactions between non-pathogenic Fusarium oxysporum V5w2 and Radopholus similis', Physiological and Molecular Plant Pathology, vol. 82, no. 4, pp. 56-63. |
en_US |
dc.identifier.issn |
0885-5765 (print |
|
dc.identifier.issn |
1096-1178 (online) |
|
dc.identifier.other |
10.1016/j.pmpp.2012.10.003 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/21581 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Elsevier |
en_US |
dc.rights |
© 2013 Elsevier. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Physiological and Molecular Plant Pathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Physiological and Molecular Plant Pathology, vol. 82, no. 4, 2013, DOI.org/10.1016/j.pmpp.2012.10.003. |
en_US |
dc.subject |
cDNA-AFLPs |
en_US |
dc.subject |
Fusarium oxysporum |
en_US |
dc.subject |
Musa spp |
en_US |
dc.subject |
Quantitative real-time polymerase chain reaction (qRT-PCR) |
en_US |
dc.subject |
Radopholus similis |
en_US |
dc.title |
Differential gene expression in East African highland bananas (Musa spp.) : interactions between non-pathogenic Fusarium oxysporum V5w2 and Radopholus similis |
en_US |
dc.type |
Postprint Article |
en_US |