dc.contributor.author |
Adelakun, Oluyemisi E.
|
|
dc.contributor.author |
Kudanga, Tukayi
|
|
dc.contributor.author |
Green, Ivan R.
|
|
dc.contributor.author |
Le Roes-Hill, Marilize
|
|
dc.contributor.author |
Burton, Stephanie G.
|
|
dc.date.accessioned |
2013-02-19T15:03:33Z |
|
dc.date.available |
2013-02-19T15:03:33Z |
|
dc.date.issued |
2012-12 |
|
dc.description.abstract |
2,6-Dimethoxyphenol is a phenolic compound that is extensively used for the measurement of
laccase activity, but is often not exploited for its potential as an antioxidant compound. Since
laccase can be used to modify phenolic antioxidants as a way of improving their activities, the
present study investigated the laccase-mediated oxidation of 2,6-dimethoxyphenol in biphasic or
homogenous aqueous-organic media for the production of compounds with higher antioxidant
capacity than the starting substrate. The main product was a dimer (m/z 305.0672), which was
further characterized as a symmetrical C-C linked 3,3’,5,5’-tetramethoxy biphenyl-4,4’-diol. In
the monophasic system, the dimer was preferentially formed when acetone was used as co34
solvent, while in the biphasic system, formation of the dimer increased as the concentration of
ethyl acetate was increased from 50 to 90 %. The dimer showed higher antioxidant capacity than
the substrate (≈ 2×) as demonstrated by standard antioxidant assays (DPPH and FRAP). These
results demonstrate that a product of the laccase-catalysed oxidation of 2,6-dimethoxyphenol can
find useful application as a bioactive compound. |
en |
dc.description.librarian |
hb2013 |
en |
dc.description.librarian |
ai2013 |
|
dc.description.sponsorship |
National Research Foundation (South Africa) |
en |
dc.description.uri |
http://www .elsevier.com/locate/procbio |
en |
dc.identifier.citation |
Adelakun, OE, Kudanga, T, Green, IR, Le Roes-Hill, M & Burton, SG 2012, 'Enzymatic modification of 2,6-dimethoxyphenol for the synthesis of dimers with high antioxidant capacity', Process Biochemistry, vol. 47, no. 12, pp. 1926-1932. |
en |
dc.identifier.issn |
1359-5113 (print) |
|
dc.identifier.issn |
1873-3298 (online) |
|
dc.identifier.other |
10.1016/j.procbio.2012.06.027 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/21113 |
|
dc.language.iso |
en |
en |
dc.publisher |
Elsevier |
en |
dc.rights |
© 2012 Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Process Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Process Biochemistry, vol. 47, Dec 2012, doi: 10.1016/j.procbio.2012.06.027. |
en |
dc.subject |
2,6- Dimethoxyphenol |
en |
dc.subject |
Antioxidant capacity |
en |
dc.subject.lcsh |
Dimers |
en |
dc.subject.lcsh |
Antioxidants |
en |
dc.subject.lcsh |
Laccase |
en |
dc.subject.lcsh |
Phenols |
en |
dc.title |
Enzymatic modification of 2,6-dimethoxyphenol for the synthesis of dimers with high antioxidant capacity |
en |
dc.type |
Postprint Article |
en |