The use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus

Du Plessis, D.H.; Van Wyngaardt, Wouter; Romito, M.; Du Plessis, M.; Maree, Sarita

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1999 Onderstepoort Journal of Veterinary Research, Volume 66, 1999
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dc.contributor.author	Du Plessis, D.H.
dc.contributor.author	Van Wyngaardt, Wouter
dc.contributor.author	Romito, M.
dc.contributor.author	Du Plessis, M.
dc.contributor.editor	Verwoerd, Daniel Wynand
dc.contributor.upauthor	Maree, Sarita
dc.date.accessioned	2012-10-01T11:48:59Z
dc.date.available	2012-10-01T11:48:59Z
dc.date.created	2012
dc.date.issued	1999
dc.description	The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.	en
dc.description.abstract	An indirect sandwich ELISA that can detect as little as 8 ng of African horsesickness virus (AHSV) was developed. Viral antigen was captured from suspension using an immobilized monoclonal antibody specific for an epitope on VP7, a protein that is a major constituent of the virus core. Egg-yolk derived chicken lgY directed against AHSV (serotype 3) was used as the secondary antibody. Since lgY and mouse lgG do not cross-react serologically, the secondary antibody was not labelled, but was instead detected with enzyme-coupled sheep antibodies directed against avian immunoglobulins. The assay recognized all nine AHSV serotypes, but not the Cascara isolate of equine encephalosis virus, a related orbivirus that also infects horses. In addition to being able to detect and quantify whole AHSV, the ELISA could show the presence of VP7 produced by recombinant baculoviruses.	en
dc.description.librarian	mn2012	en
dc.identifier.citation	Du Plessis, DH, Van Wyngaardt, W, Romito, M, Du Plessis, M & Maree, S 1999, 'The use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus’. Onderstepoort Journal of Veterinary Research, vol. 66, no. 1, pp. 25-28.	en
dc.identifier.issn	0330-2465
dc.identifier.uri	http://hdl.handle.net/2263/19926
dc.language.iso	en	en
dc.publisher	Pretoria : Agricultural Research Council, Onderstepoort Veterinary Institute	en
dc.rights	©ARC-Onderstepoort (original). ©University of Pretoria. Dept of Library Services (digital).	en
dc.subject	African horse sickness	en
dc.subject	Antibody sandwich	en
dc.subject	Chickens	en
dc.subject	Egg-yolk	en
dc.subject	Orbiviruses	en
dc.subject	AHS
dc.subject.lcsh	Veterinary medicine -- South Africa
dc.subject.lcsh	African horse sickness -- South Africa	en
dc.subject.lcsh	Veterinary virology	en
dc.subject.lcsh	Horses -- Diseases	en_ZA
dc.title	The use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus	en
dc.type	Article	en