dc.contributor.author |
Da Silva, Laura S.
|
|
dc.contributor.author |
Jung, Rudolf
|
|
dc.contributor.author |
Zhao, Zuo-yo
|
|
dc.contributor.author |
Glassman, Kimberly
|
|
dc.contributor.author |
Taylor, Janet
|
|
dc.contributor.author |
Taylor, J.R.N. (John Reginald Nuttall)
|
|
dc.date.accessioned |
2012-09-20T07:27:57Z |
|
dc.date.available |
2012-09-20T07:27:57Z |
|
dc.date.issued |
2011-07 |
|
dc.description.abstract |
To improve sorghum grain protein nutritional quality, improved sorghum lines were transformed to
suppress the synthesis of different kafirin sub-classes, or backcrossed into transgenic lines with
improved protein quality. Co-suppression of the alpha-, gamma- and delta-kafirin sub-classes and
removal of the tannin trait resulted in transgenic sorghum lines with high cooked protein digestibility
( 80%), improved Amino Acid Score (0.8) and Protein Digestibility Corrected Amino Acid Score (0.7)
compared to the non-transgenic null controls ( 50%, 0.4 and 0.2, respectively). These high protein
quality lines had a floury endosperm. They also had modified protein body structure, where the protein
bodies were irregular shaped with few to numerous invaginations and were less densely packed, with
a dense protein matrix visible around the protein bodies. When fewer sub-classes were suppressed, i.e.
gamma 1 and delta 2, the endosperm was corneous with normal protein body structure but the
improvement in cooked protein digestibility appeared to be less. Apparently, co-suppression of several
kafirin sub-classes is required to obtain high protein nutritional quality sorghum lines, but this seems to
result in floury-type grain endosperm texture. |
en_US |
dc.description.sponsorship |
The Bill and Melinda Gates Grand Challenges 9, Africa Biofortified Sorghum (ABS) Project, the South African National Research Foundation, and the Faculty Research and Innovation Committee, Faculty of Science, Tshwane University of Technology. |
en_US |
dc.description.uri |
http://www.elsevier.com/locate/jcs |
en_US |
dc.identifier.citation |
Laura S. da Silva, Rudolf Jung, Zhu-yo Zhao, Kimberly Glassman, Janet Taylor & John R.N. Taylor, Effect of suppressing the synthesis of different kafirin subclasses on grain endosperm texture, protein body structure and protein nutritional quality in improved sorghum lines, Journal of Cerial Science, vol. 54, no. 1, pp. 160-167 (2011), doi: 10.1016/j.jcs.2011.04.009. |
en_US |
dc.identifier.issn |
0733-5210 (print) |
|
dc.identifier.issn |
1095-9963 (online) |
|
dc.identifier.other |
10.1016/j.jcs.2011.04.009 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/19852 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Elsevier |
en_US |
dc.rights |
© 2011 Elsevier. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Journal of Cereal Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Cereal Science, vol 54, issue 1, July 2011, doi: 10.1016/j.jcs.2011.04.009. |
en_US |
dc.subject |
Sorghum |
en_US |
dc.subject |
Transgenic |
en_US |
dc.subject |
Protein bodies |
en_US |
dc.subject |
Digestibility |
en_US |
dc.title |
Effect of suppressing the synthesis of different kafirin subclasses on grain endosperm texture, protein body structure and protein nutritional quality in improved sorghum lines |
en_US |
dc.type |
Postprint Article |
en_US |