dc.contributor.author |
Dittrich-Schroder, Gudrun
|
|
dc.contributor.author |
Wingfield, Michael J.
|
|
dc.contributor.author |
Klein, Hildegard
|
|
dc.contributor.author |
Slippers, Bernard
|
|
dc.date.accessioned |
2012-03-19T07:13:17Z |
|
dc.date.available |
2013-01-31T10:07:45Z |
|
dc.date.issued |
2012-01 |
|
dc.description.abstract |
DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating
a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in
isolating DNA. Success of each method was scored on a 2% agarose gel after PCR of the cox 1 mitochondrial locus. A silicamembrane-
based approach was the most successful, followed by a method using a combination of incubation buffers and
amethod using magnetic beads. The method using buffers was the most cost- and time effective. Using this method, larvae
from Eucalyptus seed capsule galls could be assigned a role (parasitoid, gall former or inquiline) in the gall-inhabiting
complex. |
en |
dc.description.librarian |
nf2012 |
en |
dc.description.uri |
http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1755-0998 |
en_US |
dc.identifier.citation |
Dittrich-Schroder, G, Wingfield, MJ, Klein, H & Slippers, B 2012, 'DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps', Molecular Ecology Resources, vol. 12, no. 1, pp. 1-19. |
en |
dc.identifier.issn |
1755-098X (print) |
|
dc.identifier.issn |
1755-0998 (online) |
|
dc.identifier.other |
10.1111/j.1755-0998.2011.03074.x |
|
dc.identifier.uri |
http://hdl.handle.net/2263/18473 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Wiley-Blackwell |
en_US |
dc.rights |
© 2011 Blackwell Publishing Ltd. The definite version is available at onlinelibrary.wiley.com. This article is embargoed by the publisher until January 2013. |
en |
dc.subject |
DNA extraction |
en |
dc.subject |
Cox 1 |
en |
dc.subject |
Minute insects |
en |
dc.subject |
MtDNA barcoding |
en |
dc.subject.lcsh |
Animal genetics -- Technique |
en |
dc.subject.lcsh |
Hymenoptera -- Genetics |
en |
dc.subject.lcsh |
Wasps -- Genetics |
en |
dc.subject.lcsh |
DNA fingerprinting of animals |
en |
dc.title |
DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps |
en |
dc.type |
Postprint Article |
en |