Conclusions: The injection of two-mule Tzaneen virus protected against four-mule Tzaneen virus, but not completely against the exposure virus. The two-mule Tzaneen virus, the four-mule Tzaneen virus, and the exposure virus when injected separately or as a mixture produced reactions and an acceleration of the pulse, and no animals died. Since the two and four mule virus did not completely protect against the ephemeral fever virus, we have to conclude that these, although distinct, are to a certain extent related. Neither of the three ephemeral fever vira protected against a horse-sickness vira, and therefore it has to be concluded that there is no connection between these ephemeral fevers and horse-sickness.
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