The dual functionality of antimicrobial peptides Os and Os‐C in human leukocytes

dc.contributor.authorTaute, Helena
dc.contributor.authorBester, Megan Jean
dc.contributor.authorGaspar, Anabella Regina Marques
dc.contributor.emailhelena.taute@up.ac.zaen_ZA
dc.date.accessioned2019-05-27T07:29:23Z
dc.date.issued2019-04
dc.description.abstractAntimicrobial peptides (AMPs), Os and Os‐C, have been identified as multifunctional peptides with antibacterial, antiendotoxin, and anti‐inflammatory properties. For further development of Os and Os‐C as therapeutic peptides, it is essential to evaluate these effects in human mononuclear (MN) and polymorphonuclear (PMN) leukocytes. The cytotoxicity and the effects of both peptides on MN and PMN morphology were determined with the Alamar‐Blue assay and scanning electron microscopy, respectively. The ability of Os and Os‐C to induce reactive oxygen species (ROS) and to protect against 2,2′‐azobis(2‐amidinopropane) dihydrochloride–induced oxidative damage in both cell populations was evaluated using 2′,7′‐dichlorofluorescin diacetate (DCFH‐DA). Using fluorescently labeled peptides, the ability of the peptides to cross the cell membranes of MN and PMN was also evaluated. At the minimum bactericidal concentrations of Os and Os‐C, neither peptide was cytotoxic. Os caused morphological features of toxicity at 100 μM, entered MN cells, and also protected these cells against oxidative damage. Os‐C caused MN and PMN leukocyte activation associated with ROS formation and was unable to penetrate cell membranes, indicating extracellular membrane interactions. This study confirms that both Os and Os‐C at less than 100 μM are not cytotoxic. The MN‐specific uptake of Os identifies it as a cell‐specific cargo‐carrier peptide, with additional anti‐inflammatory properties. In contrast, the ability of Os‐C to activate MN and PMN cells implies that this peptide should be further evaluated as an AMP, which, in addition to its ability to eradicate infection, can further enhance host immunity. These novel characteristics of Os and Os‐C indicate that these AMPs as peptides can be further developed for specific applications.en_ZA
dc.description.departmentAnatomyen_ZA
dc.description.departmentBiochemistryen_ZA
dc.description.departmentGeneticsen_ZA
dc.description.departmentMicrobiology and Plant Pathologyen_ZA
dc.description.embargo2020-04-01
dc.description.librarianhj2019en_ZA
dc.description.sponsorshipThe National Research Foundation and the South African Medical Research Council.en_ZA
dc.description.urihttp://wileyonlinelibrary.com/journal/pscen_ZA
dc.identifier.citationTaute H, Bester MJ, Gaspar ARM. The dual functionality of antimicrobial peptides Os and Os‐C in human leukocytes. Journal of Peptide Science. 2019;25:e3156. https://doi. org/10.1002/psc.3156.en_ZA
dc.identifier.issn1075-2617 (print)
dc.identifier.issn1099-1387 (online)
dc.identifier.other10.1002/psc.3156
dc.identifier.urihttp://hdl.handle.net/2263/69208
dc.language.isoenen_ZA
dc.publisherWileyen_ZA
dc.rights© 2019 European Peptide Society and John Wiley & Sons, Ltd. This is the pre-peer reviewed version of the following article : The dual functionality of antimicrobial peptides Os and Os‐C in human leukocytes. Journal of Peptide Science. 2019;25:e3156. https://doi. org/10.1002/psc.3156, which has been published in final form at : http://wileyonlinelibrary.com/journal/psc.en_ZA
dc.subjectAnti-inflammatoryen_ZA
dc.subjectAntimicrobial peptide (AMP)en_ZA
dc.subjectAntioxidanten_ZA
dc.subjectCell-penetrating peptideen_ZA
dc.subjectDefensinen_ZA
dc.subjectLeukocyte activationen_ZA
dc.subject.otherHealth sciences articles SDG-03
dc.subject.otherSDG-03: Good health and well-being
dc.titleThe dual functionality of antimicrobial peptides Os and Os‐C in human leukocytesen_ZA
dc.typePostprint Articleen_ZA

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