Expression and production of soluble Mycobacterium tuberculosis H37Rv mycosin-3

Fang, Zhuo; Schubert, Wolf-Dieter; Van Pittius, Nicolaas Claudius Gey

Expression and production of soluble Mycobacterium tuberculosis H37Rv mycosin-3

dc.contributor.author	Fang, Zhuo
dc.contributor.author	Schubert, Wolf-Dieter
dc.contributor.author	Van Pittius, Nicolaas Claudius Gey
dc.contributor.email	wolf-dieter.schubert@up.ac.za	en_ZA
dc.date.accessioned	2017-05-19T09:51:03Z
dc.date.available	2017-05-19T09:51:03Z
dc.date.issued	2016-02-06
dc.description.abstract	Mycobacteria encode five type VII secretion system (T7SS) or ESX for nutrient acquisition and virulence. Mycosins are membrane-anchored components of ESX with serine protease activity but an unidentified substrate range. Establishing the substrate specificity of individual mycosins will help to elucidate individual ESX functions. Mycosin-1 and -3 orthologues from two environmental mycobacterial species, Mycobacterium smegmatis and Mycobacterium thermoresistibile, have been heterologously produced, but mycosins from Mycobacterium tuberculosis (Mtb) remain to be studied. Here we describe the successful production of Mtb mycosin-3 as a first step in investigating its structure and function.	en_ZA
dc.description.department	Biochemistry	en_ZA
dc.description.librarian	am2017	en_ZA
dc.description.sponsorship	The National Research Foundation to WDS in the form of Incentive Funding and Competitive Funding CPRR13092045495.	en_ZA
dc.description.uri	http://www.sherpa.ac.uk/romeo/issn/2405-5808/	en_ZA
dc.identifier.citation	Fang, Z, Schubert, W-D & Van Pittus, NCG 2016, 'Expression and production of soluble Mycobacterium tuberculosis H37Rv mycosin-3', Biochemistry and Biophysics Reports, vol. 5, pp. 448-452.	en_ZA
dc.identifier.issn	2405-5808
dc.identifier.other	10.1016/j.bbrep.2016.02.005
dc.identifier.uri	http://hdl.handle.net/2263/60580
dc.language.iso	en	en_ZA
dc.publisher	Elsevier	en_ZA
dc.rights	© 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CCBY-NC-ND license	en_ZA
dc.subject	ESX	en_ZA
dc.subject	Mycosin-3	en_ZA
dc.subject	Proteinproduction	en_ZA
dc.subject	Cloning	en_ZA
dc.subject	Mycobacterium tuberculosis (MTB)	en_ZA
dc.title	Expression and production of soluble Mycobacterium tuberculosis H37Rv mycosin-3	en_ZA
dc.type	Article	en_ZA