Expression and production of soluble Mycobacterium tuberculosis H37Rv mycosin-3

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Authors

Fang, Zhuo
Schubert, Wolf-Dieter
Van Pittius, Nicolaas Claudius Gey

Journal Title

Journal ISSN

Volume Title

Publisher

Elsevier

Abstract

Mycobacteria encode five type VII secretion system (T7SS) or ESX for nutrient acquisition and virulence. Mycosins are membrane-anchored components of ESX with serine protease activity but an unidentified substrate range. Establishing the substrate specificity of individual mycosins will help to elucidate individual ESX functions. Mycosin-1 and -3 orthologues from two environmental mycobacterial species, Mycobacterium smegmatis and Mycobacterium thermoresistibile, have been heterologously produced, but mycosins from Mycobacterium tuberculosis (Mtb) remain to be studied. Here we describe the successful production of Mtb mycosin-3 as a first step in investigating its structure and function.

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Keywords

ESX, Mycosin-3, Proteinproduction, Cloning, Mycobacterium tuberculosis (MTB)

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Citation

Fang, Z, Schubert, W-D & Van Pittus, NCG 2016, 'Expression and production of soluble Mycobacterium tuberculosis H37Rv mycosin-3', Biochemistry and Biophysics Reports, vol. 5, pp. 448-452.