Efficacy of a plant-produced infectious bronchitis virus-like particle vaccine in specific pathogen-free chickens

dc.contributor.authorSepotokele, Kamogelo M.
dc.contributor.authorO’Kennedy, Martha M.
dc.contributor.authorHayes, M.C.
dc.contributor.authorWandrag, D.B.R. (Daniel)
dc.contributor.authorSmith, Peter W.
dc.contributor.authorAbolnik, Celia
dc.date.accessioned2024-10-02T05:48:14Z
dc.date.available2024-10-02T05:48:14Z
dc.date.issued2023-10
dc.description.abstractInfectious bronchitis (IB) Gammacoronavirus causes a highly contagious respiratory disease in chickens that is listed by the World Organisation for Animal Health (WOAH). Its high mutation ability has resulted in numerous variants against which the commercially available live or recombinant vaccines singly offer limited protection. Agrobacterium-mediated transient expression in Nicotiana benthamiana (tobacco) plants was used here to produce a virus-like particle (VLP) vaccine expressing a modified full-length IBV spike (S) protein of a QX-like IB variant. In a challenge study with the homologous live IB QX-like virus, VLP-vaccinated birds produced S protein-specific antibodies comparable to those produced by live-vaccinated birds seroconverting with mean geometric titers of 6.8 and 7.2 log2, respectively. The VLP-vaccinated birds had reduced oropharyngeal and cloacal viral shedding compared to an unvaccinated challenged control and were more protected against tracheal ciliostasis than the live-vaccinated birds. While the results appeared similar, plant-produced IB VLPs are safer, more affordable, easier to produce and update to antigenically match any emerging IB variant, making them a more suitable alternative to IBV control than live-attenuated vaccines.en_US
dc.description.departmentProduction Animal Studiesen_US
dc.description.librarianam2024en_US
dc.description.sdgSDG-02:Zero Hungeren_US
dc.description.sdgSDG-03:Good heatlh and well-beingen_US
dc.description.sponsorshipThe Department of Science and Innovation/ National Research Foundation (DSI/NRF); and bursaries from the University of Pretoria, the DSI/NRF and the HWSETA.en_US
dc.description.urihttps://www.journals.elsevier.com/poultry-science/en_US
dc.identifier.citationSepotokele, K.M., O'Kennedy, M.M., Hayes, M.C. et al. 2023, 'Efficacy of a plant-produced infectious bronchitis virus-like particle vaccine in specific pathogen-free chickens', PoultryScience, vol. 102, no. 102953, pp. 1-9. https://DOI.org/10.1016/j.psj.2023.102953.en_US
dc.identifier.issn0032-5791 (print)
dc.identifier.issn1525-3171 (online)
dc.identifier.other10.1016/j.psj.2023.102953
dc.identifier.urihttp://hdl.handle.net/2263/98425
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2023 The Authors. This is an open access article under the CC BY license.en_US
dc.subjectInfectious bronchitisen_US
dc.subjectVaccineen_US
dc.subjectGammacoronavirusen_US
dc.subjectRespiratory diseaseen_US
dc.subjectChickensen_US
dc.subjectNicotiana benthamianaen_US
dc.subjectTobacco (Nicotiana benthamiana)en_US
dc.subjectVirus-like particle (VLP)en_US
dc.subjectSDG-03: Good health and well-beingen_US
dc.subjectSDG-02: Zero hungeren_US
dc.titleEfficacy of a plant-produced infectious bronchitis virus-like particle vaccine in specific pathogen-free chickensen_US
dc.typeArticleen_US

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