A novel nepovirus causing a chlorotic fleck disease on common bean (Phaseolus vulgaris L.)
| dc.contributor.author | Abraham, Adane | |
| dc.contributor.author | Kidanemariam, Dawit B. | |
| dc.contributor.author | Winter, Stephan | |
| dc.contributor.author | Vetten, H.J. | |
| dc.date.accessioned | 2024-01-10T05:29:58Z | |
| dc.date.available | 2024-01-10T05:29:58Z | |
| dc.date.issued | 2022-10 | |
| dc.description | DATA AVAILABILITY : The sequence data generated for this study can be found in the NCBI database (accession numbers ON148341-ON148344). | en_US |
| dc.description.abstract | Two common bean leaf samples from Ethiopia that had shown chlorotic fleck and veinal mosaic symptoms but tested ELISA-negative for known viruses were mechanically transmitted to herbaceous hosts to obtain virus isolates ET-773/4 and ET-779. Virus purification from Chenopodium quinoa systemically infected with ET-773/4 yielded icosahedral particles measuring ~ 30 nm in diameter and containing a single capsid protein of ~ 58 kDa, suggesting a nepovirus infection. Analysis of nucleotide sequences generated from RNA1 and RNA2 of the isolates indicated that they represent a distinct virus species in the genus Nepovirus. Surprisingly, the most closely related sequence in the GenBank database was that of Hobart nepovirus 3, an incompletely described metagenomic sequence obtained from honey bees in Tasmania. This new nepovirus from Ethiopia is provisionally named "bean chlorotic fleck virus". | en_US |
| dc.description.department | Forestry and Agricultural Biotechnology Institute (FABI) | en_US |
| dc.description.department | Plant Production and Soil Science | en_US |
| dc.description.librarian | hj2023 | en_US |
| dc.description.sdg | None | en_US |
| dc.description.uri | https://link.springer.com/journal/705 | en_US |
| dc.identifier.citation | Abraham, A., Kidanemariam, D.B., Winter, S. et al. A novel nepovirus causing a chlorotic fleck disease on common bean (Phaseolus vulgaris L.). Archives of Virology 167, 2057–2061 (2022). https://doi.org/10.1007/s00705-022-05507-6. | en_US |
| dc.identifier.issn | 0304-8608 (print) | |
| dc.identifier.issn | 1432-8798 (online) | |
| dc.identifier.other | 10.1007/s00705-022-05507-6 | |
| dc.identifier.uri | http://hdl.handle.net/2263/93881 | |
| dc.language.iso | en | en_US |
| dc.publisher | Springer | en_US |
| dc.rights | © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2022. The original publication is available at : http://link.springer.comjournal/705. | en_US |
| dc.subject | Common bean (Phaseolus vulgaris L.) | en_US |
| dc.subject | Nucleotide sequences | en_US |
| dc.subject | Random-PCR | en_US |
| dc.subject | Polymerase chain reaction (PCR) | en_US |
| dc.subject | Ethiopia | en_US |
| dc.subject | Electron microscopy | en_US |
| dc.subject | Biological test | en_US |
| dc.subject | Antiserum production | en_US |
| dc.title | A novel nepovirus causing a chlorotic fleck disease on common bean (Phaseolus vulgaris L.) | en_US |
| dc.type | Postprint Article | en_US |