A novel nepovirus causing a chlorotic fleck disease on common bean (Phaseolus vulgaris L.)
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Date
Authors
Abraham, Adane
Kidanemariam, Dawit B.
Winter, Stephan
Vetten, H.J.
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
Two common bean leaf samples from Ethiopia that had shown chlorotic fleck and veinal mosaic symptoms but tested ELISA-negative for known viruses were mechanically transmitted to herbaceous hosts to obtain virus isolates ET-773/4 and ET-779. Virus purification from Chenopodium quinoa systemically infected with ET-773/4 yielded icosahedral particles measuring ~ 30 nm in diameter and containing a single capsid protein of ~ 58 kDa, suggesting a nepovirus infection. Analysis of nucleotide sequences generated from RNA1 and RNA2 of the isolates indicated that they represent a distinct virus species in the genus Nepovirus. Surprisingly, the most closely related sequence in the GenBank database was that of Hobart nepovirus 3, an incompletely described metagenomic sequence obtained from honey bees in Tasmania. This new nepovirus from Ethiopia is provisionally named "bean chlorotic fleck virus".
Description
DATA AVAILABILITY : The sequence data generated for this study can be found in the NCBI database (accession numbers ON148341-ON148344).
Keywords
Common bean (Phaseolus vulgaris L.), Nucleotide sequences, Random-PCR, Polymerase chain reaction (PCR), Ethiopia, Electron microscopy, Biological test, Antiserum production
Sustainable Development Goals
None
Citation
Abraham, A., Kidanemariam, D.B., Winter, S. et al. A novel nepovirus causing a chlorotic fleck disease on common bean (Phaseolus vulgaris L.). Archives of Virology 167, 2057–2061 (2022). https://doi.org/10.1007/s00705-022-05507-6.