Two different banana NPR1-like coding sequences confer similar protection against pathogens in Arabidopsis

dc.contributor.authorYocgo, Rosita Endah
dc.contributor.authorCreissen, Gary
dc.contributor.authorKunert, Karl J.
dc.contributor.authorChikwamba, R.K. (Rachel Kerina)
dc.contributor.emailkarl.kunert@fabi.up.ac.zaen_ZA
dc.date.accessioned2016-11-29T07:58:18Z
dc.date.available2016-11-29T07:58:18Z
dc.date.issued2012-12
dc.description.abstractDifferences between the coding sequences of two banana homologues of the Non-expressor of Pathogenesis-Related 1 (NPR1) genes (MNPR1A and MNPR1B) were investigated as a possible cause for the differential activity of the two genes. Each of the MNPR1 coding sequences were expressed under the control of the cauliflower mosaic virus 35S promoter/terminator sequences in the transgenic Arabidopsis npr1-2 mutant. These MNPR1-expressing plants were then exposed to either the biotrophic oomycete Hyaloperonospora arabidopsidis, the necrotrophic fungus Botrytis cinerea, or the hemi-biotrophic bacterial pathogen Pseudomonas syringae. Expression of either MNPR1A or MNPR1B increased Pathogenesis-related 1 (PR-1) transcription in a similar manner in mutant plants and reduced pathogen growth, restoring resistance of the Arabidopsis npr1-2 mutant plants to pathogens. Sequence differences between MNPR1A and MNPR1B coding sequences did not affect MNPR1activity, suggesting a possible role for the involvement of other regulatory sequences in differential MNPR1 gene expression.en_ZA
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_ZA
dc.description.departmentPlant Scienceen_ZA
dc.description.librarianhb2016en_ZA
dc.description.sponsorshipRockefeller Foundation and the University of Pretoria.en_ZA
dc.description.urihttp://link.springer.com/journal/12042en_ZA
dc.identifier.citationYocgo, R.E., Creissen, G., Kunert, K.J. & Chikwamba, R.K. Two different banana NPR1-like coding sequences confer similar protection against pathogens in Arabidopsis. Tropical Plant Biology. (2012) 5: 309. doi:10.1007/s12042-012-9112-y.en_ZA
dc.identifier.issn1935-9756 (print)
dc.identifier.issn1935-9764 (online)
dc.identifier.other10.1007/s12042-012-9112-y
dc.identifier.urihttp://hdl.handle.net/2263/58302
dc.language.isoenen_ZA
dc.publisherSpringeren_ZA
dc.rights© Springer Science+Business Media New York 2012. The original publication is available at : http://link.springer.com/journal/12042.en_ZA
dc.subjectBanana NPR1-like sequencesen_ZA
dc.subjectBotrytis cinereaen_ZA
dc.subjectHyaloperonospora arabidopsidisen_ZA
dc.subjectPR proteinsen_ZA
dc.subjectPseudomonas syringaeen_ZA
dc.subjectSequence dissimilarityen_ZA
dc.subjectNon-expressor of Pathogenesis-Related 1 (NPR1)en_ZA
dc.titleTwo different banana NPR1-like coding sequences confer similar protection against pathogens in Arabidopsisen_ZA
dc.typePostprint Articleen_ZA

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