Two different banana NPR1-like coding sequences confer similar protection against pathogens in Arabidopsis
dc.contributor.author | Yocgo, Rosita Endah | |
dc.contributor.author | Creissen, Gary | |
dc.contributor.author | Kunert, Karl J. | |
dc.contributor.author | Chikwamba, R.K. (Rachel Kerina) | |
dc.contributor.email | karl.kunert@fabi.up.ac.za | en_ZA |
dc.date.accessioned | 2016-11-29T07:58:18Z | |
dc.date.available | 2016-11-29T07:58:18Z | |
dc.date.issued | 2012-12 | |
dc.description.abstract | Differences between the coding sequences of two banana homologues of the Non-expressor of Pathogenesis-Related 1 (NPR1) genes (MNPR1A and MNPR1B) were investigated as a possible cause for the differential activity of the two genes. Each of the MNPR1 coding sequences were expressed under the control of the cauliflower mosaic virus 35S promoter/terminator sequences in the transgenic Arabidopsis npr1-2 mutant. These MNPR1-expressing plants were then exposed to either the biotrophic oomycete Hyaloperonospora arabidopsidis, the necrotrophic fungus Botrytis cinerea, or the hemi-biotrophic bacterial pathogen Pseudomonas syringae. Expression of either MNPR1A or MNPR1B increased Pathogenesis-related 1 (PR-1) transcription in a similar manner in mutant plants and reduced pathogen growth, restoring resistance of the Arabidopsis npr1-2 mutant plants to pathogens. Sequence differences between MNPR1A and MNPR1B coding sequences did not affect MNPR1activity, suggesting a possible role for the involvement of other regulatory sequences in differential MNPR1 gene expression. | en_ZA |
dc.description.department | Forestry and Agricultural Biotechnology Institute (FABI) | en_ZA |
dc.description.department | Plant Science | en_ZA |
dc.description.librarian | hb2016 | en_ZA |
dc.description.sponsorship | Rockefeller Foundation and the University of Pretoria. | en_ZA |
dc.description.uri | http://link.springer.com/journal/12042 | en_ZA |
dc.identifier.citation | Yocgo, R.E., Creissen, G., Kunert, K.J. & Chikwamba, R.K. Two different banana NPR1-like coding sequences confer similar protection against pathogens in Arabidopsis. Tropical Plant Biology. (2012) 5: 309. doi:10.1007/s12042-012-9112-y. | en_ZA |
dc.identifier.issn | 1935-9756 (print) | |
dc.identifier.issn | 1935-9764 (online) | |
dc.identifier.other | 10.1007/s12042-012-9112-y | |
dc.identifier.uri | http://hdl.handle.net/2263/58302 | |
dc.language.iso | en | en_ZA |
dc.publisher | Springer | en_ZA |
dc.rights | © Springer Science+Business Media New York 2012. The original publication is available at : http://link.springer.com/journal/12042. | en_ZA |
dc.subject | Banana NPR1-like sequences | en_ZA |
dc.subject | Botrytis cinerea | en_ZA |
dc.subject | Hyaloperonospora arabidopsidis | en_ZA |
dc.subject | PR proteins | en_ZA |
dc.subject | Pseudomonas syringae | en_ZA |
dc.subject | Sequence dissimilarity | en_ZA |
dc.subject | Non-expressor of Pathogenesis-Related 1 (NPR1) | en_ZA |
dc.title | Two different banana NPR1-like coding sequences confer similar protection against pathogens in Arabidopsis | en_ZA |
dc.type | Postprint Article | en_ZA |